Streptomyces coelicolor의 발아과정 중 RNA와 단백질 합성의 주기적 변화

Periodical Changes of RNA and Protein Syntheses During the Germination of Streptomyces coelicolor

  • 이지훈 (인하대학교 이과대학 생물학과) ;
  • 한홍의 (서울대학교 분자미생물학 연구센터)
  • 발행 : 1997.03.01

초록

성장인자가 포함되지 않은 무기염 액체배지(ISP-4)에서 Streptomyces coelicolor A3(2)의 포자가 발아할 때, 성장인자의 주기적인 요구성이(양 등,1993_ 단백질과 RNA 합성과 어떤 관계를 가지고 있는지를 규명하고자 하ㅣ였다. 발아는 10시간 정도 걸렸으며 이때 성장인자의 요구성이 2시간 주기로 반복되는 것을 재입증하였다. 포자의 크기는 시간에 따라 증가하였으나, 포자수는 표준 평판계수법에서 감소하였다. 포자 집단은 생리적으로 살아있거나, 휴면 중인 포자와 죽은 포자로 구분될 수 있었다. 이러한 발아과정에서 포자를 acridine orange(AO)와 iodonitrotetrazolium chloride(INT)로 염색하여 형광현미경으로 관찰하였을 때 RNA와 단백질도 일정한 주기를 갖고 합성되었으며, 이 주기성은 성장인자의 요구 주기와 거의 일치하였다. 이로써 발아 초기에 포자 집단은 성장인자 중에서 특히 단백질 합성과 관련된 물질인 아미노산이 주기적으로 요구되고 있음을 토론하였다.

This study was to elucidate the relation between the periodical requirement of growth factors(Yang et al., 1993) and the synthesis of RNA and protein during the germination of Streptomycn coefic%r A3(2) in mineral liquid medium(ISP-4) without addition of growth factors. As results, The germination time was about 10 hr, and meanwhile, periodical nutritional requirement was verified to be repeated with interval of 2 hr. Spore size was enlarged with time but its number was rather decreased. Spore could be deviJed into viable, dormant, and dead state. In such a germination process it was found that RNA and protein were being synthesized periodically when spores were stained with AO and INT methods and observed under the fluorescence microscope. Those syntheses were coincided with the period of nutritional requirement. Hence, it was discllssed that spore population in early germination would need amino acids related to protein synthesis.

키워드

참고문헌

  1. 인하대학교 석사학위 논문 한천과 폴리아크릴아마이드 젤 배지상에서 Streptomyces spp. 배양특성의 비교 백지호
  2. 한국미생물학회지 v.31 Streptomyces coelicolor A3(2)의 유도기에서 성장 인자 요구성의 교대 현상 양 문;한홍의
  3. 인하대학교 박사학위 논문 Streptomyces coelicolor의 발아과정 중 아미노산 요구시기의 Damped Cscillation 양상 양 문
  4. Microbial ecology: fun-damentals and applications Atals, R. M.;R. Bartha
  5. Appl. Microbiol. v.23 Microflora of soil as viewed by transmission electron mi-croscopy Bae, Gabriel;Ben Koopman
  6. Appl. Environ. Mi-crobiol v.43 Tetrazolium reduction-malachite green method fof assessing the viability of filamentous bacteria in active sludge Bitton;Gabriel;Ben Koopman
  7. Streptomyces coel-icolor A3(2). Cell. v.52 At least three different RNA polymerase holoenzyme direct tran-scription of the agarase gene(dagA) Buttner, M.;A.M. Smith;M.J. Bibb
  8. Appl. Environ. Mi-crobiol. v.57 Viable butnonculturable bacteria in drinking water Byrd, J.J.;H. Xu;R.R. Colwell
  9. Biology of in-dustrial microorganisms Demain, A.L.;N.A. Solomon
  10. Limnol. Oceanogr v.23 Seasonal changes in respiratory enzyme activity and productivity in Lake Washington microplankton Devol, A.M.;T.T. Packard
  11. Appl. Environ. Microbiol. v.46 Mala-chite green INT(MINT) method for determining active bac-teria in sewage Dutton, R.J.;G. Bitton;B. Koopman
  12. FEMS microbiol. Lett. v.14 Characterization fo RNA species synthesized during germination and vegetative growth of Streptomyces antibioticus Guijarro, J.A.;J.E. Suarez;J.A. Salas;C. Hardis-son
  13. J. Microbiol. v.29 Pattern of protein degradation during germina-tion of Stretomyces antibioticus Guijarro, J.A.;J.E. Suarez;J.A. Salas;C. Hardis-son
  14. Bacteriol. Rev. v.41 Bacterial respira-tion Haddock, B.A.;C.W. Jones
  15. Appl. Environ, Microbiol. v.33 Use of nu-cleopore filter for counting bacteria by fluorescence mi-croscopy Hobbie, J.E.;R.J. Daley;S. Jasper
  16. Microbiological Reviews v.58 Use of fluochromes for direct enumeration of total bacteria in environmental samples: Past and present Kepner, R.L.;J.R. Pratt
  17. Appl. Environ. Microbiol. v.54 A simple rapid method for enumerating total viable and metabolically ac-tive bacteria in groundwater King, L.K.;B.C. Parker
  18. Bergey's manual of systematics bacteriology v.4 Streptomyces and related genera Locci, R.;S.T. (et al.)
  19. Advanced in microbial ecology v.6 Starvation-survival of beterotrophs in the marine environment Morita, R.Y.;K. C. Marshall (ed.)
  20. Appl. Microbiol. v.11 Influence of agar on the morphology and pigmentation of Streptomyces Okami, Y.;S. Arima;M. Suzuki
  21. Appl. Environ. Microbiol. v.58 Use of a fluorescent redox probe for direct visualization fo activity respiring bacteria Rodriguez, G.G.;D. Phipps;K. Ishiguro;H.F. Ridg-way
  22. Appl. Environ. Microbiol. v.43 Improved method for determination of respiring individual microorganisms' irr na-tural waters Tabor, P.S.;R.A. Neihof