Journal of Microbiology

  • 제35권3호
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  • Pages.228-233
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  • 1997
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Amino acid substitutions conferring cold-sensitive phenotype on the yeast MTF1 gene

  • 발행 : 1997.09.01
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초록

The MTF1 gene of Saccharomyces cerevisiae encodes a 43 kDa MITOCHONDRIAL RNA polymerase specificity factor which recognizes mitochondrial promoters to initiate correct transcription. To better understand structure-function of the MTF1 gene as well as the transcription mechanism of mitochondrial RNA polymerase, two cold-sensitive alleles of the MTF1 mutation were isolated by plasmid shuffling method after PCR-based random mutagenesis of the MTF1 gene. The mutation sites were analyzed by nucleotide sequencing. These cs phenotype mtf1 mutants were respiration competent on the nonfermentible glycerol medium at the permissive temperature, but incompetent at 13.deg.C. The cs phenotype allele of the MTF1, yJH147, encoded an L146P replacement. The other cs allele, yJH148, contained K179E and K214M double replacements. Mutations in both alleles were in a region of Mtflp which is located between domains with amino acid sequence similarities to conserved regions 2 and 3 of bacterial s factors.

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참고문헌

  1. Genetics v.116 A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains Alani, E.;L. Cao;N. Kleckner
  2. J. Biol. Chem. v.262 In vitro characterization of the yeast mitochondrial promoter using single-base substitution mutants Biswas, T.K.;B. Ticho;G.S. Getz
  3. Mol. Gen. Genet. v.197 A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance Boeke, J.D.;F. LaCroute;G.R. Fink
  4. Gene v.74 New yeast-E. coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites Gietz, R.D.;A. Sugino
  5. Cell v.69 SSL2, a suppressor of a stem-loop mutation in the HIS4 leader encodes the yeast homolog of human ERCC-3 Gulyas, K.D.;T.F. Donahue
  6. Annu. Rev. Biochem. v.57 Structure and function of bacterial sigma factors Helmann, J.D.;M.J. Chamberlin
  7. Gene v.57 A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli Hoffman, C.;F. Winston
  8. J. Bacteriol. v.153 Transformation of intact yeast cells treated with alkali cations Ito, H.;Y. Fukuda;K. Murata;A. Kimura
  9. J. Biol. Chem. v.266 The yeast mitochondrial RNA polymerase specificity factor, MTF1, is similar to bacterial б factors Jang, S.H.;J.A. Jaehning
  10. Transcription: mechanisms and regulation Mechanisms of mitochondrial transcription Jang, S.H.;J.A. Jaehning;R.C. Conaway(ed.);J.W. Conaway(ed.)
  11. J. Bacteriol. v.174 Effects of amino acid substitutions in the -10 binding region of ${\sigma}^E$ of Bacillus subtilis Jones, C.H.;M. Tatti;C.P. Moran, Jr.
  12. J. Mol. Biol. v.235 A promoter melting region in the primary s factor of Bacillus subtilis: identification of functionally important aromatic amino acids Juang, Y.L.;J.D. Helmann
  13. J. Biol. Chem. v.261 Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase Kelly, J.L.;A.L. Greenleaf;I.R. Lehman
  14. Biochemistry v.28 Characterization of the Escherichia coli transcription factor ${\sigma}^{70}$: Iocalization of a region involved in the interaction with core RNA polymerase Lesley, S.A.;R.R. Burgess
  15. J. Cell. Mol. Biol. v.1 A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction Leung, D.W.;E. Chen;D.V. Goeddel
  16. Mol. Gen. Genet. v.214 A nuclear gene essential for mitochondrial replication suppresses a defect of mitochondrial transcription in Saccharomyces cerevisiae Lisowsky, T.;G. Michaelis
  17. J. Bacteriol. v.174 The ${\sigma}^{70}$ family: sequence conservation and evolutionary relationship Lonetto, M.;M. gribskov;C.A. Gross
  18. J. Mol. Biol. v.53 Calcium-dependent bacteriophage DNA infection Mandel, M.;A. Higa
  19. J. Biol. Chem. v.269 Release of the yeast mitochondrial RNA polymerase specificity factors from transcription complexes Mangus, D.A.;S.H. Jang;J.A. Jaehning
  20. Mol. Cell. Biol. v.9 Use of yeast nuclear DNA sequences to define the mitochondrial RNA polymerase promoter in vitro Marczynski, G.T.;P.W. Schultz;J.A. Jaehning
  21. Cell v.51 Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7 Master, B.S.;L.L. Stohl;D.A. Clayton
  22. Yeast v.8 A rapid method for localized mutagenesis of yeast genes Muhlrad, D.;R. Hunter;R. Parker
  23. Methods Enzymol. v.101 One-step gene disruption in yeast Rothstein, R.J.
  24. Proc. Natl. Acad. Sci. v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;S. Nicklen;A.R. Coulson
  25. Mol. Cell. Biol. v.15 A Saccharomyces cerevisiae mitochondrial transcription factor, sc-mtTFB, shares features with sigma factors but is functionally distinct Shadel, G.S.;D.A. Clayton
  26. Methods in yeast genetics Sherman, F.;G. Fink;J. Hicks
  27. Methods Enzymol. v.194 In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast Sikorski, R.S.;J.D. Boeke
  28. J. Mol. Biol. v.98 Detection of specific sequences among DNA fragments separated by gel electrophoresis Southern, E.M.
  29. Mol. Cell. Biol. v.14 Glucose repression of yeast mitochondrial transcription: Kinetics of derepressioin and role of nuclear genes Ulery, T.L.;S.H. Jang;J.A. Jaehning
  30. J. Mol. Biol. v.235 Probing the information content of Escherichia coli ${\sigma}^{70}$ region 2.3 by combinatorial cassette mutagenesis Waldburger, C.;M.M. Susskind
  31. J. Biol. Chem. v.263 Two forms of RPO41-dependent RNA polymerase Wilcoxen, S.E.;C.R. Peterson;C.S. Winkley;M.J. Keller;J.A. Jaehning
  32. J. Bacteriol. v.174 A mutant ${\sigma}^{70}$ with a small deletion in conserved region 3 of б has reduced affinity for core RNA polymerase Zhou, Y.N.;W.A. Walter;C.A. Gross
  33. Nucleic Acids Res. v.24 Sequence homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the eukaryotic lineage Cermakian, N.;T.M. Ikeda;R. Cedergren;M.W. Gray
  34. J. Mol. Biol. v.215 Changes in conserved region 2 of Escherichia coli ${\sigma}^{70}$ affecting promoter recognition Waldburger, C.;T. Gardella;W. Wong;M.M. Susskind