Journal of Microbiology

  • 제35권3호
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  • Pages.177-180
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  • 1997
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Eveluation of line probe assay in detecting rifampicin resistance of mycobacterium tuberculosis

  • 발행 : 1997.09.01
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초록

The purpose of this study was to evaluate the efficiency of Line Probe Assay (LiPA) in detecting the rpoB gene mutation of clinically isolated Mycobacterium tuberculosis (MTB) and to compare the level of resistance to the various rifamycins with their mutation sites. The mutation in the rpoB gene was found in 84 (97.6%) out of 86 rifampicin (RMP) resistant strains as determined by LiPA. No mutation was observed in 2 RMP resistant strains and in any of 38 RMP susceptible strains tested. Only one of 3 strains with .DELTA.5/R5, one of 2 strains with .DELTA.3, and one of 3 strains with .DELTA.2/R2 LiPA profile showed a slightly lower level of resistance to the rifapentine than the other strains. Although we could not find correlations between mutation sites in the rpoB gene and the level of susceptibility to the various rifamycins, the LiPA is recommended as a fast screening tool for detection of RMP resistant MTB.

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참고문헌

  1. Science v.263 inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis Banerjee, A.;E. Dubnau;A. Quemard;V. Balasubramanian;K.S. Um;T. Wilson;D. Collins;de G. Lisle;Jr.W.R. Jacobs
  2. J. Antimicrob. Chemother. v.35 Mutation position and type of substitution in the β-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis Bodmer, T.;G. Zurcher;P. Imboden;A. Telenti
  3. Trends in Microbiol. v.2 Mycobacterium tuberculosis: drug-resistance mechanism Cole, S.T.
  4. Antimicrob. Agents. Chemother. v.39 Ribosylation by Mycobacterial strains as a New mechanism of rifampin inactivation Dabbs, E.R.;K. Yazawa;Y. Mikami;M. Miyaji;N. Morisaki;S. Iwasaki;K. Furihata
  5. Tubercle Lung. Dis. v.76 Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay De Beenhouwer, H.;Z. Lhiang;G. Jannes;W. Mijs;L. Machtelinckx;R. Rossau;H. Traore;F. Portaels
  6. Biochem Biophys Acta v.145 The specific inhibition of the DNA-directed RNA synthesis by rifamycin Hartmann, G.;K.O. Honikel;F. Knussel
  7. Lancet v.344 Implications of multidrug resistance for the future of shortcourse chemotherapy of tuberculosis: a molecular study Heym, B.;N. Honore;C. Truffot-Pernot;A. Banerjee;C. Schurra;Jr.W.R. Jacobs
  8. J. Clin. Microbiol. v.32 Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase β subunit in rifampicin resistant Mycobacterium tuberculosis strains from New York City and Texas Kapur, V.;L.L. Li;S. Iordanescu;M.R. Hamrick;A. Wanger;B.N. Kreiswirth;J.M. Musser
  9. Tubercle Lung. Dis. v.73 Drug resistance of Mycobacterium tuberculosis in Korea Kim, S.J.;Y.P. Hong
  10. TB Weekly v.29 KRM-1648 may be superior rifampin-resistant TB Moghazeh, S.
  11. J. Infect. Dis. v.171 Molecular mechanism of multiple drug resistance in clinical isolates of Mycobacterium tuberculosis Morris, S.;G.H. Bai;P. Suffys;L. Portillo-Gemez;M. Fairchok;D. Rouse
  12. Mol. Microbiol. v.10 The rpsL gene and streptomycin resistance in single and multiple drug-resistant strains of Mycobacterium tuberculosis Nair, J.;D.A. Rouse;G.H. Bai;S.L. Morris
  13. TB Weekly v.6 69-bp fragment in rpoB gene predicts resistant Ohno, H.
  14. J. Korean Soc. Microbiol. v.31 Detection of rifampicin resistant Mycobacterium tuberculosis by Line Probe Assay Park, Y.K.;S.H. Cho;M.S. Shim;G.H. Bai;S.J. Kim;C.Y. Song
  15. Nature Medicine v.2 Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercule bacillus Scorpio, A.;Y. Zhang
  16. Antimicrob. Agents. Chemother. v.40 Characterization of rpsL and rrs mutation in streptomycin-resistant Mycobacterium tuberculosis isolates from diverse geographic localities Sreevatsan, S.;X. Pan;K.E. Stockbauer;D.L. Williams;B.N. Kreiswirth;J.M. Musser
  17. Antimicrob. Agents. Chemother. v.38 Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes, and characterization of quinolone resistance mutations Takiff, H.E.;L. Salazar;C. Guerrero;W. Phillip;W.M. Huang;B. Kreisworth;S.T. Cole;Jr.W.R. Jacobs;A. Telenti
  18. Lancet v.341 Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis Telenti, A.;P. Imboden;F. Marchesi;D. Lowrie;S. Cole;M.J. Colston;L. Matter;K. Schopfer;T. Bodmer
  19. Antimicrob. Agents. Chemother. v.37 Direct, automated detection of rifampin resistant Mycobacterium tuberculosis conformation polymorphism analysis Telenti, A.;P. Imboden;F. Marchesi;T. Schmidheini;T. Bodmer
  20. Rev. Infect. Dis. v.5 Rifampin: Mechanisms of action and resistance Wehrli, W.
  21. J. Clin. Microbiol. v.33 Direct genotypic detection of Mycobacterium tuberculosis refampin resistance in clinical specimens by using single-tube heminested PCR Whelen, A.C.;T.A. Felmlee;J.M. Hunt;D.L. Williams;G.D. Roberts;L. Stockman;D.H. Persing
  22. Mol. Microbiol. v.19 ahpC, a gene involved in isoniazid resistance of the Mycobacterium tuberculosis complex Wilson, T.M.;D.M. Collins
  23. Nature v.358 The catalase-peroxidase gene and isoniazid resistance of Mycobacterium tuberculosis Zhang, Y.;B. Heym;B. Allen;D. Young;S. Cole