Journal of Microbiology

  • 제35권1호
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  • Pages.40-46
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  • 1997
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Nucleotide sequence analysis of a second set of the polyketide synthase .betha.-ketoacyl synthase and chain length factor genes from the salinomycin-producing streptomyces albus

  • 발행 : 1997.03.01
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초록

The pWHM220 cosmid with a 24-kb insert cloned from Streptomyces albus ATCC 21838 induces the biosynthesis of a polysther antibiotic similar to salinomycin in Streptomyces invidans. We have analyzed this region by DNA sequencing as well as Southern blot hybridization with type I and type II polyketide synthase (PKS) probes. Surprisingly, we found another set of type II SKS genes only 10-kb from the original PKS genes, salABCDE. The DNA sequence revealed two complete open reading frames (ORFs) named salB2 and salC2, and one partial ORF that does not resemble any known DNA or deduced protein sequence. The salC2 should code for chain length determining factor while the deduced amino acid sequence encoded by salB2 exhibits high similarity to .betha.-ketoacyl synthase from different PKS gene clusters. The highest identity was found for .betha.-keetoacyl synthases from S. argillaceus (MtmP. 59.1% identity), the mithramycin producer and from S. venezuelae ISP5230 (JadA, 52.3% identity), the jadomycin producer. The SalC2 protein clearly resembles its counterparts in order aromatic PKS gene clusters that are believed to influence the length of the polyketide chain. The highest identities observed were to that of S. argillaceus (MtmK, 62.3%) and S. venezuelae ISP 5230 (JadB, 55.1%) proteins, Moreover, the deduced amino acid sequences of the salB2 and salC2 products were 29.0% identical.

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참고문헌

  1. J. Mol. Biol. v.215 Basic local alianment search tool Altchul S.F.;W. Gish;W. Miller;E.W. Myers;D.J. Lipman
  2. Annu. Rev. Microbiol. v.31 Tndem genetic duplication in phage and bacteria Anderson, R.P.;J.R. Roth
  3. Mol. Gen Genet v.234 Characterization of actI-homologous DNA encoding polyketide synthase genes from the monensin producer Streptomyces cinnamonensis Arrowsmith, T.J.;F. Malpartida;D.H. Sherman;A. birch;D.A. Hopwood;J. A. Robinson
  4. Science v.262 Enzymatic synthesis of a bacterial polyketide from acetyl and malonyl coenzyme A Ben Shen;C. Richard Hutchinson
  5. Gene v.142 Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenoliein Bibb, M.J.;D.H. Sherman;S. Omura;D.A. Hopwood
  6. Gene v.30 The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences Bibb, M.J.;P.R. Findlay;M.W. Johnson
  7. EMBO J. v.8 Analysis of the nucleotide sequence of the Streptomyces glaucescens tem1 genes provides key information about the enzymology of polyketide antibiotic biosynthesis Bibb, M.J.;S. Biro;H. Motamedi;J.F. Collins;C.,R. Hutchinson
  8. Microbiology Production of antibiotics salinomycin by introduction foa gene cluster containing type Ⅱ polyketide synthase genes from Strreptomyces albus ATCC 21838 into Streptomyces lividans Borell, Chris W.;Y.G. He;H.J. Kwon;C.G. Hyun;K. Fumio;J.W. Suh;H.J. Jin;H.G. Floss;C.R. Hutchinson
  9. Mol. Microbiol. v.4 Spore colour in Streptomyces coelicolor A3(2) involves the development tally regulated synthesis of a compound biosynthetically related to polyketide antibiotics Davids, N.K.;K.F. Chater
  10. Nucleic Acids Res. v.12 A comprehensive set of sequence analysis programs for the VAX Devereux, J.;P. Haeberli;O. Smithies
  11. Science v.252 Modular organizaion of genes required for complex polyketide biosynthesis Donadio, S.;M.J. Staver;J. B. McAlpine;S.J. Swanson;L. Katz
  12. J. Biol. Chem. v.264 Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin Fernandez-Moreno, M.A.;E. Martinez;L. Boto;D.A. Hopwood;F. Malpartida
  13. Nature Genet v.3 Identification of protein coding regions by database similarity search Gish, W.;D.J. States
  14. Gene v.151 Characterization of the Streptomyces peucelius ATCC 29050 genes encoding doxorubiein polyketide synthase Grimm, A.;Madduri, K.;Ali, A.;Hutchinson, C.R.
  15. Microbiology v.140 Cloning and characterization of polyketide synthase genes for jadomycin B bioshnthesis in Streptomyces venezulelae ISP5230 Han, L.:Yang, K.;Ramalingan, E.;Mosher, R.H.;L.C. Vining
  16. Genetics and Ⅱ olecular biology of industrial microorganisms Toward an understanding of the programming of aromatic polyketide synthases a genetics-driven approach Hopwood, D.A.;C. Khosla;D.H. Sherman;M.J. Bibb;S. Ebert-Khosla;E.-S. Kim;R. McDaniel;W.P. Revill;R. Torres;T.-W. Yu;G. D. Hegeman(ed.);R.H. Baltz(ed.);P.L. Skatrud(ed.)
  17. Annu. Rev. Genet. v.24 Molecular genetics of polyketides and its comparison to fatty acid biosynthesis Hopwood, D.A.;D.H. Sherman
  18. Annu. Rev. Microbiol. v.49 Polyketide synthase gene manipulation: A structure-function approach in engineering novel antibioties Hutchinson, C.R.;I. Ikeda, I.
  19. J. bacteriol. v.175 Genetic constructon and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier prpteins Khosla, C.;R. McDaniel;S. Ebert-Khosla;R. Torres;D.H. Sherman;M.J. Bibb;D.A. Hopwood
  20. J. Bacteriol. v.176 Analysis of type Ⅱ β-ketoacyl synthase specificity in Streptomyces coelicolor A3(2) by trans complementation of actinorhodin synthase mutants Kim, E.S.;D.A. Hopowood;D.H. Sherman
  21. Gene v.141 Sequences of the oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus Kim, E.S.;M.J. Bibb;M.J. Bulter;D.A. Hopwood;D.H. Sherman
  22. Gene v.172 Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin Lombo, F.;G. Blanco;E. Fernandez;C. Mendez;J.A. Salas
  23. FEMS Microbiol. Lett. v.99 Two different β-lactamase genes are present in Streptomyces cacaoi Magdalena, J.;M. Forsman;M.V. Lenzini;A. Brans;J. Dusart
  24. Science v.262 Engineered biosynthesis of novel polyketides McDaniel, R.;S. Ebert-Kosla;D.A. Hopwood;C. Khosla
  25. J. Am. Chem. Soc. v.15 Engineered biosynthesis of novel polyketides: manipulation and analysis of an aromatic polyketide synthase with unproven catalytic specificities McDaniel, R.;S. Ebert-Kosla;D.A. Hopwood;C. Khosla
  26. Nature v.375 Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits McDaniel, R.;S. Ebert-Kosla;D.A. Hopwood;C. Khosla
  27. Gene v.61 A new shuttle vector, pKC505, for streptomycetes: its use in the cloning of three different spiramycin-resistance genes from a Streptomyces ambofaciens library Richardson, M. A.;S. Kuhstoss;P. Solenberg;N. Schaus;R.N. Rao
  28. Molecular cloning: a laboratory manual(2nd ed.) Sambrock, J.;E.F. Fritsch;T. Maniatis
  29. Proc. Natl. Acad. Sci. USA v.74 DNA sequencing with chain terminating inhibitors Sanger, F.;S. Nicklen;A.R. Coulson
  30. Proc. Natl. Acad. Sci. USA v.93 The biosynthesis gene cluster for the polyketide immunosuppressant rapamycin Schweche, T.;J.F. Aparicio;I. Molnar;A. Konig;L.E. Khaw;S.F. Haydock;M. Oliynyk;P. Caffrey;J. Cortes;J.J.B. Lester;G.A. Bohm;J. Staunton;P.F. Leadley
  31. J. bacteriol. v.174 Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterologous genes from a different polyketide pathway Sherman, D.H.;E.-S. Kim;M.J. Bibb;D.A. Hopwood
  32. EMBO J. v.8 Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tu22 Sherman, D.H.;F. Malpartida;M.J. Bibb;H.M. Kieser;D.A. Hopwood
  33. Gene v.33 Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp19 and pUC19 vectors Yanisch-Perron, C.;J. Vieira;J. Messing
  34. Microbiology v.141 Ectopic expression of the Streptomyces coelicolor whiE genes for polyketide spore pigment synthesis and their interaction with the act genes for actimorhodin biosynthesis Yu, T.W.;D.A. Hopwood
  35. J Bacteriol. v.176 Cloningm sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus Yu, T.W.;M.J. Bibb;W.P. Revill;D.A. Hopwood