한국발생생물학회지:발생과생식 (Development and Reproduction)
- 제1권1호
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- Pages.29-36
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- 1997
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- 2465-9525(pISSN)
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- 2465-9541(eISSN)
자주복 (Takifugu rubripes) 정자의 동결보존
Cryopreservation of Tiger Puffer (Takifugu rubripes) Sperm
초록
Experiments were performed to study the effects of diluents, cryoprotectant, equilibration time, thawing temperature and addition of BSA and egg yolk. Among the various diluents, Alsever's solution was the best for sperm cryopreservation. A combination of Alsever's solution and 15% ethylene glycol showed the better results than others did. Sperm activity indection and survival rate gradually decreased with the equilibration time. The appropriate thawing temperature was 30
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