Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Induction of Cytochrome P450 by Ionones in Liver Lobes of Sprague Dawley Rats

Ionone류에 의한 랫드의 간엽별 cytochrome P450 유도 특성에 관한 연구

  • 구희경 (한국화학연구소 안전성연구센터) ;
  • 정태천 (한국화학연구소 안전성연구센터) ;
  • 천영진 (중앙대학교 약학대학) ;
  • 윤철호 (배재대학교 생화학과) ;
  • 노정구 (한국화학연구소 안전성연구센터) ;
  • 최인경 (덕성여자대학교 약학대학)
  • Published : 1997.12.01
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Abstract

Inductive effects of cytochrome P450 2B1 by $\alpha$- and $\beta$-ionone were characterized in individual liver lobes of male Sprague Dawley rats. When rats were administered ionones orally at 100, 300, and 600 mg/kg for 24 hr, cytochrome P450 2B1 was induced dose-dependently in liver S-9 fractions as measured by P450 2B-specific monooxygenases and Western immunoblotting. The activity of P450 1A- and P450 2B-specific monooxygenases was differentially expressed in each lobe of normal liver. In addition, the monooxygenase activity was induced by $\alpha$- and $\beta$-ionone with different potency in each lobe of the liver. Our present results indicate that the different induction of P450s by $\alpha$- and $\beta$-ionone in each lobe may explain different susceptibilities of rat liver lobes to certain hepatotoxicants which require metabolic activation for their toxicity and that $\alpha$- and $\beta$-ionone may be useful model inducers of P450 2B1 in studying the toxic mechanism of certain toxicants which may require the metabolic activation by P450.

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References

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