Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

Involvement of the Enhancement of Natural Killer Cell Activity on the Anti-Cancer Effect of Red Gingseng during Rat Hepatocarcinogenesis

랫드의 간압발생과정에서 홍삼의 항암효과와 자연살해세포의

  • 강경선 (서울대학교 수의과대학 공중보건학교실) ;
  • 이영순 (서울대학교 수의과대학 공중보건학교실)
  • Published : 1997.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to examine the anti-cancer effect of Red Ginseng in the DENGalN-PH-induced hepatic tumor model system in rats. One hundred of male SPF Sprague-Dawley rats(6weeks old) were randomly divided into five groups. Rats in groups 1, 2, 3, and 4 were administered to diethylnitrosamine intraperitoneally 200 mg/kg body weight for the caner initiation. Rats in group 5 were given to saline as a control. On two weeks after cancer initiation, rats in groups 1 and 3 were fed on diet containing 0.01% of acethylaminofiuorene(AAF) which is strong cancer-promotor for 6 weeks, while rats in groups 2 and 4 were fed on water containing 0.05% of phenobarbital which is weak cancer.promotor for 6 weeks. Rats in groups 1 and 2 were treated with diet containing 3% of Red Ginseng for six weeks(from 9th week till 15th week after cancer initiation). Rats in all groups were necropsied time-sequencially at 8, 15, and 36 weeks. The hepatic lesions of rat treated with carcinogens expressed glutathione S-transferase placental form(GST-P) at 8 week. The GST-P positive foci of rats treated with AAF were larger than that of any other rats, while the GST-P positive foci of rats treated with AAF and red ginseng were significantly decreased. This anti-cancer effect of Red ginseng might be involved in the enhacement of natural killer cell activity. To know whether there is direct relationship between Red Ginseng and natural killer cell activity, the activity of natural killer cell was examined after treatment AAF, AAF+Red ginseng and Red ginseng only, respectively. Comparing with natural killer cell activity in AAF-treated group, natural killer cell activity was significantly activated in AAF+ Red ginseng-treated group. This indicated that Red ginseng might enhance natural killer activity after treatment carcinogen in rats. These results suggested that Red ginseng might have a cancer prevention ability by promoting natural killer cell activity during hepatocarclnogenesis.

Keywords

References

  1. Science v.221 Dietary carcinogens and anticarcinogens Ames B.N.
  2. Laboratory Investigation v.44 Spongiosis hepatis Specific chages of the perisinusoidal liver cells induced in rats by n-nitrosomorpholine Bannasch P.;Bloch M.;Zerban H.
  3. J. of Immunology v.134 Direct evidence for the role of LGL in the inhibition of experimental tumor metastases Barlozzari T.;Leonhardt J.;Wiltrout R.H.;Herberman R.B.;Reynolds C.W.
  4. Cancer Research v.46 Expression of H-ras and c-myc protooncogenes in isolated r-glutamyl transpeptidase-positive rat hapatocytes and in hepatocellular carcinomas induced by diethylnitrosamine Beer D.G.;Schwarz M.;Sawada N.;Pitot H.C.
  5. Cancer Research v.143 Change in the host lymphocyte subsets during chemical carcinoginesis Brodt P.;Lala P.K.
  6. Korean J. Ginseng Sci. v.14 Effects ginseng saponin fractions and lipopolisaccharide on the tumoricidal activity of mouse macrophage Choi S.U.;Jung N.P.;Kim S.C.
  7. Immunology Today v.9 A definition of natural killer cells Fitzgerald-Bocarsly P.;Herberman R.;Hercend T.;Hiserodt J.;Kumar V.;Wigzell H.
  8. J. of Experimental Medicine v.174 Mechanism of target cell recognition by natural killer cells: Characterization of a novel triggering molecule restricted to CD3- large granular lymphocytes Frey J.L.;Bino T.;Kantor R.R.S.;Segal D.M.;Giardina S.L.
  9. Carcinogenesis v.9 Cancer promoting potential of different strains of Fusarlum moniliforme in a short term cancer initiation/promotion assay Gelderblom W.C.A.;Marasas W.F.O.;Jaskiewicz K.;Combrinck S.;van Schalkwyk D.J.
  10. J. of Immunological Methods v.29 Purification of human T and B cells by a discontinuous density gradient of percoll Gutierrez C.;Bernabe, R.R.;Vega J.;Kreisler M.
  11. J. of Immunology v.127 Definitive evidence that natural killer(NK) cells inhibit exprrimental tumor metastasis in vivo Hanna N.;Burton R.C.
  12. JNCI v.65 Role of natural killer cells in the destruction of ciruclating tumor emboli Hanrra N.;Fidler L.J.
  13. Korean J. Ginseng Sci. v.13 Effects of panax ginseng and sodium ascorbate (Vitamin C) treatment on cancer cell growth Hwang W.I.;Son H.S.;Ji R.H.;Baik N.G.
  14. Gann v.75 Amplification and over-expression of the c-myc gene in morris hepatomas Hayashi K.;Reiko M.;Sugimura T.
  15. Ann. Rev. Immunol. v.4 Mechanism of cytotoxicity by natural killer(NK) cells Herbaman R.B.;Reynolds C.W.;Ortaldo J.R.
  16. Advances in Cancer Research v.27 Natural cell mediated immunity Herberman R.B.;Holden H.T.
  17. Science v.214 Natural killer cells: Their role in defenses against disease Herberman R.B.;Ortaldo J.R.
  18. AJP v.121 Laminin inhibits the recognition of tumor target cells by murine natural killer(NK) and natural cytotoxic(NC) lymphocytes Hiserodt J.C.;Laybourn K.A.;Varani J.
  19. Korean J. Ginseng Sci. v.16 Hwang W.I.
  20. Hepatology v.15 Immunohistochemical study of extracellular matrix in acute galactosamine hepatitis in rats Jonker, A.M.;Dijkhuis F.W.J.;Boes A.;Hardonk M.J.;Grond J.
  21. Korean J. of Lab. Ani. Sci v.4 Medium-term bioassay models for environmental carcinogens - Two step liver and multi-organ carcinogenesis protocols Ito N.;Tsuda H.;Hasegaw R.;Tatematsu M.;Imaida K.;Asamoto M.
  22. Korean J. Ginseng Sci. v.15 Effects of ginseng saponin fraction and cyclophosphamide on the tumoricidal activity of mouse macrophage and the antitimor effect Jeon H.K.;Kim S.C.;Jung N.P.
  23. Korean Vet. Res. v.32 Isolation and morphological characterization of natural killer cell in the Sprague-dawley(SD) rats Kang K.S.;Lee Y.S.
  24. Korean J. Toxicol. v.8 The study on carcinogenicity of coptis rhizome and croton tiglium L. Kang K.S.;Cho J.J.;Kim B.H.;Kim H.O.;Shin D.J.;Lee Y.S.;Lim Y.K.
  25. Kor. J. Food Hygiene v.6 The comparison of efficacy of glutathione S-transferase placental form positive and iron-resistnant lesions in the detection of hepatocarcinogens Kang K.S.;Kim H.C.;Lee Y.S.
  26. Korean J. Toxicol. v.5 Effects of Ganoderma Lucidum extract on the develpment of rat liver preneoplastic lesions Kim J.S.;Lee Y.S.
  27. Korean J. Ginseng Sci. v.13 The effect of ginseng saponin fractions on NK activity in mice Kim M.N.;Jung N.P.
  28. Korean J. Toxicol. v.4 Inhibitory effects of red ginseng extract on preneoplastic nodule formation in the rat liver observed by rapid bioassay of carcinogenesis Kim Y.B.;Lee Y.S.
  29. Korean J. Ginseng Sci. v.15 Effects of ginseng components on immunotoxicity of cyclosphophamide Kim Y.S.;Kang K.S.;Kim S.I.
  30. Proceedings of the 2nd life science symposium on biomedical research in red ginseng Korea Ginseng & Tobacco Research institute
  31. Proceedings of the 3rd life science symposium on biomedical research in red ginseng Korea Ginsing & Tobacco Reseach institute
  32. J. of Immunology v.137 Natural killer cells: Definition of a cell type rather than a function Lanier L.L.;Phillips J.H.;Hackett J. JR.;Tutt M.;Kmmar V.
  33. Cancer Research v.45 Suppression of natural killer and lymphocyte functions associated with carcinogen-induced premalignant hyperplastic nodules in rat liver Leu R.W.;Norton T.R.;Herriott M.J.;Ringer D.P.;Kearns R.J.
  34. Korean J. Toxicol. v.5 Inhibitory effects of red ginseng extract on development of pepsinogen 1 decreased pyloric glands in rat stomach Lim C.H.;Hur G.H.;Lee Y.S.
  35. Kor. J. Food Hygiene v.5 no.4 Promotive Effects of Several Synthetic Hormones Using Short-Term Carcinogen Screening Method Lim Y.K.;Kang K.S.;Lee Y.S.
  36. Biochemical and Biophysical Research Communication v.119 Expressions of the c-Ha-ras and c-myc genes in rat liver tumors Makino R.;Hayashi K.;Sato S.;Sugimura T.
  37. Report on the 1991 special survey for cause of death National Statistical Office Republic of Korea
  38. K.H.M. v.3 An experimental study on the effect of raw ginseng, white ginseng and red ginseng on immune response in mice Oh O.S.;Ko B.H.;Song I.B.
  39. Korean J. Ginseng Sci. v.14 Induction of phenotypic reverse transformation by ginsenosides in cultured cancer cells Ota T.;Yamamoto K.F.;Kohno H.;Odashima S.
  40. Korean J. Ginseng Sci. v.16 Effects of ginseng saponin on DNA strand breaks and replication inhibition by benzo(a)pyrene in CHO-K1 cells Park J.K.;Park K.H.
  41. Immunology Today v.8 Natural killer activity: the definition of a function rather than a-cell type Reynolds C.W.;Ortaldo J.R.
  42. J. of Immunology v.127 Natural killer activity in the rat Ⅱ.Analysis of surface antigens on LGL by flow cytometry Reynolds C.W.;Sharrow S.O;Ortaldo J.R.;Herberman R.B.
  43. J. of Immunology v.127 Natural killer(NK) cell activity in the rat Ⅰ. Isolation and characterization of the effector cells Reynolds C.W.;Timonen T.;Herberman R.B.
  44. J. of Immunology v.136 Natural killer cell activity in the rat Ⅴ. The circulation patterns and tissue localization of peripheral blood large granular lymphocytes(LGL) Rolstad B.;Herberman R.B.;Reynolds C.W.
  45. Nature v.222 Platinum compounds, a new class of potent anti-tumor agents Rosenberg B.;Van Camp L.;Trasko J.E.;Mansour V.H.
  46. Immuological Rev. v.44 Morphological and functional characterization of isolated effector cells responsible for human natural killer activity to fetal fibroblasts and to cultured cell line targets Saksela E.;Timonen T.;Ranki A.;Hayry
  47. J. of Immunology v.143 Rat natural killer cells syntheseze fibronectin. Possible involvement in the cytotoxic funciton Santoni A.;Gismondi A.;Morrone S.;Procopio A.;Modesti A;Scarpa S.;Frati L.
  48. Immunology v.10 Target structures involved in natural kiling(NK): Characteristics, Distribution, and Candidate molecules Storkus W.J.;Dawson J.R.
  49. Advance in Immunology v.47 Biology of natural killer cells Trinchieri G.
  50. Pathology of tumours in laboratory animals v.1 Turusov V.S.;Mohr U.
  51. Nature v.300 Effects of a cloned cell line with NK activity on bone marrow transplants Warner J.F.;Gunther D.
  52. J. of Immunology v.132 Separation and isolation of rat natural killer(NK) cells from T cells with monoclonal antibodies Woda B.A.;Mcfadden M.L.;Welsh R.M.;Bain K.M.
  53. Korean J. Ginseng Sci. v.14 Anti tumor substance from Panax ginseng roots Yamamoto H.;Katano M.;Matsunaga H.
  54. Cancer Detection Prev. v.6 Anticarcinogenic effect of longterm oral adiministration of red ginseng on new born mice exposed to various chemical carcinogens Yun T.K.;Yun Y.S.;Han I.H.
  55. Korean J. Ginseng Sci. v.14 Preventive effect of ginseng intake on various human cancers: A case-control study Yun T.K.;Choi S.Y.
  56. Korean Biochem. J. v.18 Effect of red ginseng on natural killer cell activity in mice with lung adenoma induced by urethan and benzo(a)pyrene Yun Y.S.;Jo S.K.;Moon H.S.;Kim Y.J.;Oh Y.R.;Yun T.K.