Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
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CHEMILUMINESCENCE STUDIES ON THE BIOLOGICAl, INTERACTION BETWEEN SUPEROXIDE ANION RADICAL AND NITRIC OXIDE PRODUCED BY PHORBOI, ESTER-STIMULATED RAW264.7 MACROPHAGES

  • Lee, Hong (Department of Chemistry, Wonkwang University) ;
  • Pae, Hyun-Ock (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Jun, Chang-Duk (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Kwak, Hyun-Jeong (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Park, Rae-Kil (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Yoo, Ji-Chang (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Lee, Seog-Jae (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Kim, Myung-Sun (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Kim, Hae-Song (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Choi, Byung-Min (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) ;
  • Chung, Hun-Taeg (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University)
  • Published : 1997.06.01
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Abstract

The rapid and spontaneous interaction between superoxide anion radical and nitric oxide to yield the potent oxidants. peroxynitrite artion and peroxynitrous acid, was investigated in phorbol myristate acetate(PMA)-stimulated RAW264.7 macrophases by means of lucigenin- or luminol-enhanced chemiluminescence method. When RAW264.7 macrophages were stimulated by PMA. peroxynitrite-induced chemiluminescence was clearly observed. To prove observed chemiluminescencc due to the reaction between superoxide anion radical and nitric oxide produced by RAW264.7 macrophases, N-nitrosoglutathione (GSNO), a nitric oxide-releasing compound. superoxide dismutase(SOD), an enzyme removing superoxide anion radical by dismutating superoxide artion radical to hydrogen peroxide, and N-acethyl cysteine(NAC), a scarvenging reagent both superoxide artion radical and nitric oxide, were added in the cell system. Peroxynitrite- induced chemilumincscence was increased by exogenous addition of GSNO. whereas observed chemiluminescence was decreased by SOD and NAC. These results suggest that PMA-stimulated RAW264.7 macrophages produce both superoxide anion radical and nitric oxide to form peroxynitrite.

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