Effects of Fetal Bovine Serum(FBS), Calf Serum(CS) and Human Serum(HS) on In Vitro Maturation of Porcine Follicular Oocytes

FBS, CS 및 HS가 돼지난포란의 체외성숙에 미치는 영향

  • 임정훈 (충남대학교 농과대학 동물자원학부) ;
  • 한만희 (충남대학교 농과대학 동물자원학부) ;
  • 서길웅 (충남대학교 농과대학 동물자원학부)
  • Published : 1997.12.01

Abstract

This study was conducted to investigate the effects of fetal bovine serum(FBS), calf serum(CS) and human serum(HS) on in vitro maturation of porcine follicular oocytes. The results obtained were summarized as follows : 1. The maturation rates of oocytes cultured in medium containing FBS 5, 10, 20 and 30% were 47.0, 63.5, 48.4 and 43.2%, respectively. There were significantly higher than those of non-treated group(25.3%). And the highest maturation rate was the 10% treatment. 2. The maturation rates of oocytes cultured in medium containing CS 5, 10, 20 and 30% were 55.2, 56.6, 59.4 and 46.5%, respectively. There were significantly higher than those of non-treated group(25.3%). And the highest maturation rate was the 20% treatment. 3. The maturation rates of oocytes cultured in medium containing HS 5, 10, 20 and 30% were 74.5, 78.2, 73.1 and 68.6%, respectively. There were significantly higher than those of non-treated group(29.6%). And the highest maturation rate was the 10% treatment.

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