BMB Reports
- Volume 30 Issue 5
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- Pages.337-341
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- 1997
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- 1976-670X(eISSN)
Phospholipase D Is Not Involved in Rho A-Mediated Activation of Stress Fiber Formation
- Leem, Sun-Hee (Biomolecule Research Group, Korea Basic Science Institute) ;
- Shin, In-Cheol (Biomolecule Research Group, Korea Basic Science Institute) ;
- Kweon, Soo-Mi (Biomolecule Research Group, Korea Basic Science Institute) ;
- Kim, Seung-Il (Biomolecule Research Group, Korea Basic Science Institute) ;
- Kim, Jae-Hong (Laboratory of Molecular & Cellular Genetics, Institute of Environment & Life Science, Hallym University) ;
- Ha, Kwon-Su (Biomolecule Research Group, Korea Basic Science Institute)
- Published : 1997.09.30
Abstract
In order to investigate the role of a small GTP-binding protein RhoA in lysophosphatidic acid (LPA)-induced stress fiber formation, C3 ADP-ribosyltransferase was prepared by expressing in E. coli and then applied to Rat-2 fibroblasts. C3 transferase isolated from E. coli was as effective as the toxin from Clostridium botulinum in ADP-ribosylation of RhoA. Incubation of the cells with C3 transferase for 2 days induced ADP-ribosylation of RhoA by a dose-dependent manner, with a sub-maximal induction at