Journal of fish pathology (한국어병학회지)
- Volume 10 Issue 2
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- Pages.153-164
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- 1997
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- 1226-0819(pISSN)
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- 2233-5412(eISSN)
Studies on characteristics and pathogenicity of Yersinia sp. Isolated from cultured olive flounder, Paralichthys olivaceus
양식넙치에서 분리한 Yersinia sp.의 일부 특성과 병원성에 관한 연구
- Lee, Keun-Kwang (Department of Environment Preservation, Kumsung Environment College) ;
- Kim, Young-Gill (Department of Fish Pathology, Kunsan National University) ;
- Lee, Jae-Chang (Department of Environment Preservation, Kumsung Environment College)
- Published : 1997.12.30
Abstract
Characteristics and pathogenicity of Yersinia sp. strain isolated from diseased cultured olive flounder, Paralichthys olivaceous were studied. The causative organisms were identified as Yersinia sp. by biochemical and biophysical characteristics. The strain was named KF-1, and it showed the optimal growth rate at pH 9.0 and 1% NaCl. Total cell protein peptide bands of the Yersinia sp. KF-1 were between 14.4-100.6 Kd in molecular weight by the electrophoretic analysis, and a total of 28 bands appeared. The band found at 32.8 Kd in molecular weight was the major one of electrophoretic phase. In the pathogenicity test of the isolate to the flounder injecting with
양식넙치에 분리한 Yersisnia sp. 의 일부 특성과 병원성을 조사하였다. 넙치의 입주변 및 체표에 궤양을 유발한 원인균의 생화학적 생물물리학적 특성을 조사한 결과 이들 균은 Yersisnia sp. (KF-1)로 동정 되었다. 이들 분리균주의 발육 최적 pH는 9.0이었고, 최적 NaCl농도는 1% 이었다. Yersinia sp. (KF-1)의 전체세포를 SDS-PAGE로 분석한 결과 세포를 구성하고 있는 단백질의 분자량은 14.0-100.6kd로써 총 28개의 peptide 밴드로 구성되어 있으며, 32.8kd의 분자량을 갖는 밴드가 주밴드로 생각된다. 병원성 시험에서 KF-1을