Applied Biological Chemistry
- Volume 40 Issue 5
- /
- Pages.380-387
- /
- 1997
- /
- 2468-0834(pISSN)
- /
- 2468-0842(eISSN)
Cloning and Sequencing of the pelCl Gene Encoding Pectate Lyase of Erwinia carotovora subsp. carotovora LY34
Erwinia carotovora subsp. carotovora LY34에서 pelCI 유전자 클로닝
- Lim, Sun-Tech (Department of Agricultural Chemistry, Gyeongsang National University) ;
- Park, Yong-Woo (Department of Agricultural Chemistry, Gyeongsang National University) ;
- Yun, Han-Dae (Department of Agricultural Chemistry, Gyeongsang National University)
- Published : 1997.10.31
Abstract
Phytopathogenic Erwinia carotovora subsp. carotovora (Ecc) LY34 causes plant tissue maceration by secretion of pectinolytic enzymes such as pectate Iyase (PL) existed as multiple isoenzyme form. Genomic DNA from Ecc LY34 was digested with Sau3Al and ligated into the BamHI site of pBluescript ll
Pectate lyase isoenzymes을 분비하는 Erwinia carotovorn subsp. carotovora LY34는 식물조직을 연화시키는 연부균이다. 이 균주로부터 게놈 DNA를 분리하여 Sau3Al 제한효소로 부분 절단한 다음 pBluescript