Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Growth Inhibition of Listeria monocytogenes by Pure Compound Isolated from Extract of Morus alba Linne Bark

상백피 추출물중 Listeria monocytogenes 증식억제 물질의 분리 및 효과

  • An, Eun-Young (Department of Food Science and Technology, Chonbuk National University) ;
  • Han, Ji-Sook (Department of Food Science and Technology, Chonbuk National University) ;
  • Shin, Dong-Hwa (Department of Food Science and Technology, Chonbuk National University)
  • Published : 1997.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The crude extract of Morus alba Linne bark which showed strong antimicrobial activity on Listeria monocytogenes was fractionated by some solvents and applied column chromatography for purification of active component. By silica gel column chromatography, active fraction (F-5') was purely isolated and confirmed their strong bactericidal effect on Listeria monocytogenes tested at level of 100 and 300 ppm in tryptic soy broth. The cell wall observed by SEM was abnormal after treatment of F-5'.

상백피를 75% 에탄올로 추출하여 얻은 조 추출물을 다시 용매별로 순차 분획 후 Listeria monocytogenes 5균주에 대하여 증식저해 효과를 비교한 후 항균활성 물질을 확인하였다. 활성이 있는 획분을 1차 및 2차 column chromatography를 통하여 활성 물질을 순수 분리하여 활성 물질 획분(F-5')을 얻었고 이 획분을 tryptic soy broth에 100 및 300 ppm을 첨가하여 Listeria monocytogenes를 증식시킨 결과 배양시간에 따라 뚜렷한 균수의 감소가 관찰되어 높은 살균효과가 인정되었으며 처리된 균체의 세포적의 비정상적인 형태가 전자현미경을 통하여 관찰되었다.

Keywords