Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Inhibition of Topoisomerase-mediated DNA Cleavage by Lycoperdon perlatum

말불버섯 추출물의 Topoisomerase 저해 효과

  • Park, Mi-Jung (Biochemistry Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Cho, Kang-Jin (Biochemistry Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Jung-Bong (Biochemistry Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Dong-Hern (Biochemistry Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Yang-Sub (Applied Microbiology Division, National Institute of Agricultural Science and Technology) ;
  • Seok, Soon-Ja (Applied Microbiology Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Sun-Yeou (National Sericulture and Entomology Research Insitute, RDA) ;
  • Hwang, Young-Soo (Biochemistry Division, National Institute of Agricultural Science and Technology, RDA)
  • 박미정 (농촌진흥청 농업과학기술원 생화학과) ;
  • 조강진 (농촌진흥청 농업과학기술원 생화학과) ;
  • 김정봉 (농촌진흥청 농업과학기술원 생화학과) ;
  • 김동헌 (농촌진흥청 농업과학기술원 생화학과) ;
  • 김양섭 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 석순자 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 김선여 (농촌진흥청 농업과학기술원 잠사곤충연구소) ;
  • 황영수 (농촌진흥청 농업과학기술원 생화학과)
  • Published : 1997.10.01
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Abstract

In the course of searching for anticancer agents from 32 mushrooms, it was found that methanol extract of Lycoperdon perlatum showed inhibitory effect on topoisomerase II-mediated DNA cleavage. This active methanol extract was sequentially fractionated with hexane, chloroform, n-buthanol and water. Among the solvent-fractionated extracts, $1\;{\mu}g/mL$ hexane fraction of L. perlatum inhibited on topoisomerase II-mediated DNA cleavage. The effect of hexane fraction of L. perlatum was dose- and reaction time-dependent. The hexane fraction of L. perlatum was found to have inhibitory activity on relaxation assay of DNA topoisomerase I. The hexane fraction of cultured L. perlatum, however, had no inhibitory effect on either type of topoisomerase.

26속 32종의 버섯의 topoisomerase II 작용 억제여부를 검색한 결과 말불버섯이 topoisomerase II 작용을 억제하며 그 유효 성분이 핵산분획물에 존재함을 확인하였다. 말불버섯의 핵산분획물은 linear DNA와 open circular DNA를 생성시켰으며 농도와 반응시간에 의존적인 반응 양상을 나타냈다. 또한 말불버섯의 핵산분획물은 topoisomerase I의 작용도 억제하였다. 그러나 배양한 말불버섯의 균사체는 topoisomerase 작용에 아무런 영향을 미치지 않았다.

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