Yeast Cell Wall Lytic Enzyme Produced by Dicyma sp. YCH-37 II. Effect of Culture Conditions and Pretreatment of Yeast on the Enzyme Activity

Dicyma sp. YCH-37이 생산하는 효모세포벽 용해효소 II. 효소활성에 미치는 기질 효모의 배양조건 및 전처리 효과

We examined some properties of yeast cell wall lytic enzyme produced by Dicyma sp. YCH-37. Several metal ions, reducing reagents, and chemical modifiers have little effects on the lytic activity, except guanidine-HCl. Yeast cells of early log phase were more susceptible to the enzyme than those of stationary phase, and heat-treated cells were more easily lysed than intact living ones. Yeast cells pretreated with organic solvents such as butanol and acetone were more susceptible to the enzyme than intact living ones. Yeast cells cultured in Yeast extract-Malt extract medium containing 0.5 M ammonium sulfate were easily lysed by the lytic enzyme, and yeast cells cultured without shaking were more easily lysed by the enzyme than those with shaking. When SDS, ${\beta}-mercaptoethanol$, Triton X-100, sodium sulfite, and KCl were added to enzyme reaction mixture each, lysis of yeast cells was more effective.

Dicyma sp. YCH-37이 생산하는 효모세포벽 용해효소의 성질을 검토한 결과, 각종 환원제와 금속이온에 대체로 안정하였고, guanidine-HCl을 제외한 여러 화학수식제에 대해서도 안정하였다. 배양시간, 전처리 및 배양조건에 따른 영향을 검토한 결과, 정지기 및 사멸기에 있는 효모보다는 대수증식기의 효모, 그리고 생효모에 비해 열처리된 효모가 더 잘 용균되었다. Butanol, acetone 등의 유기용매로 처리된 효모가 그렇지 않은 효모보다 용균도가 좋았으며, 0.5 M ammonium sulfate가 함유된 Yeast extract-Malt extract 배지에서 생육한 효모, 그리고 진탕배양한 효모보다 정치배양한 효모가 용균효소에 의해 더 잘 용균되었다. SDS, Triton X-100, ${\beta}-mercaptoethanol$, potassium chloride, sodium sulfite 등의 화학수식제를 효소반응액에 첨가하였을 때 기질 효모는 더 잘 용균되었다.