Journal of The Association for Neo Medicine (제3의학)
- Volume 2 Issue 1
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- Pages.25-33
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- 1997
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- 1226-0932(pISSN)
Genetic Analysis study of Sasang Constitution Classification by RFLP
RFLP법을 이용한 사상체질의 유전적 분석 연구
- Cho, Dong-Wuk (Korea Institute of Oriental Medicine) ;
- Cho, Hwang-Sung (Korea Institute of Oriental Medicine)
- Published : 1997.01.25
Abstract
In Sasang medicine, humen are classified into four constitutions which are Taeyang, Soyang. Taeum and Soum. Depending on each different constitution, the clinical and pharmacological application for the same disease might be different. In this study, genomic DNA of different constitutions(Taeum, Soyang and Soum) were analyzed by Restriction Fragment Length Polymorphism(RFLP) to provide scientific and objective references for Sasang classification. The DNA polymorphism for each constitution detected as differences in the length of DNA fragments, after digestion with restriction enzyme Hae III, was investigated using YNH24 as DNA probe. The allele size of Taeum, Soyang and Soum group detected by YNH24 ranged from 1.3 to 3.8 kb, 1.5 to 3.9 kb and 1.3 to 4.6 kb, respectively. However, the allele size distribution of YNH24 loci of different constitutions was shown to be too variable to be classified as 3 different constitution groups investigated. For further study, it is suggested that the number of each constitution samples for RFLP analysis should be increased and statistical analysis of the allele size distribution should be carried out.