BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Cytotoxic and Apoptotic Effects of Echinomycin on Murine Leukemia Cells

  • Kim, Tae-Ue (Department of Medical Technology, College of Health Science, Yonsei University) ;
  • Yang, Se-Hwan (Department of Medical Technology, College of Health Science, Yonsei University) ;
  • Kim, Soo-Kie (College of Medicine, Yonsei University)
  • Received : 1996.04.20
  • Published : 1996.11.30
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Abstract

A number of anticancer-chemotherapeutic agents induce cell death through the process of apoptosis. Effects of echinomycin, an anticancer agent on cancer progression, were investigated in P388 murine leukemia cells. First, according to the results of cytotoxicity measurement. $IC_{50}$ of echinomycin was 1.12 nM, a relatively lower value than the other examined anticancer agents, mitomycin-C and etoposide Second, the DNA fragmentation assay for echinomycin-treated cells exhibited that echinomycin was able to induce apoptosis in a shorter period of time and with a lower dose than mitomycin-C or etoposide. The data of DNA fragmentation were quite comparable to those of cytotoxicity measurement. Finally we showed that mitogen-activated protein (MAP) kinase, a key protein in cell mitosis, was translocated into the nucleus from the cytosol after treatment with echinomycin. These findings suggest that a MAP kinase-related process may be involved in apoptosis induced by echinomycin.

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