Clinical and Experimental Reproductive Medicine
- Volume 23 Issue 3
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- Pages.357-366
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- 1996
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Metabolism of Calcium in the Oocyte Maturation of Rat
흰쥐의 난자성숙에 있어서의 칼슘의 대사
- Hong, Soon-Gab (Department of Biology, Chungbuk National University) ;
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Lee, Joon-Yeong
(Department of Biology, Chungbuk National University)
- Published : 1996.12.31
Abstract
The present experiments aimed to investigate the metabolism of calcium during oocyte maturation in rat. The concentration of free calcium and calmodulin in oocytes was measured respectively by using of fluo-3/AM and FITC with microscope fluorescence spectrometer. The ultrastructural localization of calcium precipitates in oocytes was observed with the transmission electron microscope. Cumulus-free immature oocytes(GV-oocyte) were cultured in vitro through 15 hours. The free calcium concentration in GV oocyte was