Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
권호 표지

Establishment and Characterization of Cell Lines Derived from Squamous Cell Carcinoma of the Head and Neck

두경부 편평상피세포암 세포주의 수립 및 특성

  • Kim Kwang-Hyun (Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Seoul National Universitiy) ;
  • Chung Phil-Sang (Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University) ;
  • Park Hyun-Min (Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University) ;
  • Rhee Chae-Seo (Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Seoul National Universitiy) ;
  • Park Jae-Gahb (Cancer Research Center, SNU Cell Line Bank)
  • 김광현 (서울대학교 의과대학 이비인후과학교실) ;
  • 정필상 (단국대학교 의과대학 이비인후과학교실) ;
  • 박현민 (단국대학교 의과대학 이비인후과학교실) ;
  • 이재서 (서울대학교 의과대학 이비인후과학교실) ;
  • 박재갑 (서울대학교 암연구소, 암세포주은행)
  • Published : 1996.11.01
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Abstract

We have characterized 4 human squamous carcinoma cell lines established from the larynx and hypopharynx area. All the cell lines were cultured in RPMI-1640 medium. During the growth they showed monolayer adherence pattern in culture flask. They showed tonofilament on transmission electromicroscopy which is characteristic of squamous cell epithelium. DNA finger-printing using Hinf-l proved them to be originated from different beings. Flow cytometric analysis revealed them to show aneuploidy. Immunohistochemical staining for cytokeratin was done using CK1, CK8.13, CK19 and CAM5.2 antibody, and produced various patterns of positivity. All the cell lines showed varying degrees of tumorigenecity in athymic nude mice when injected subcutaneously, but only heterotransplanted SNU-1041 cell line showed continuous tumor growth. Histopathologic findings of the heterotransplanted tumors were identical to those of the original tumors of patients. This study suggests that establishment of many different squamous cell lines might bestow great capability in researches of the head and neck cancer.

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