Rapid Determination of the Maximum Specific Growth Rates of Nitrogen Oxidizing Bacteria by Fed-Batch Experiments

Fed-Batch 실험장치(實驗裝置)를 이용한 질산화(窒酸化) 미생물(微生物)들의 최대(最大) 성장율(成長率)의 결정(決定)에 관한 실험적(實驗的) 연구(硏究)

Nitrification reaction consists of two reactions: nitritification which oxidizes ammonia nitrogen to nitrite nitrogen and nitratification which oxidizes nitrite nitrogen to nitrate nitrogen. Each reaction is carried out by Nitrosomonas and Nitrobacter, respectively. The effective maximum growth rates for both bacteria have to be determined to design aeration tank whenever the aeration tanks have to nitrify ammonia nitrogen in influent. And these values are very important to use mathematical models such as IAWPRC model to simulate nitrification in activated sludge. There are several methods to determine these valves, however, the Fed-Batch experiments can determine these values within 72 hours. In this study, the mathematical equations and experimental procedures for Fed-Batch test are presented. Also, the experimental data and reported values are compared. The estimated mean values of maximum specific growth rates for Nitrosomonas and Nitrobacter are $0.5010day^{-1}$ and $0.6704day^{-1}$, respectively.