Tetrodotoxin Occurrence in Ciliated Protozoa and Possible Bacterial Role in its Toxification

  • Do Hyung Ki (School of Bioscience and Food Technology, Handong University) ;
  • MAEDA Masachika (Nansei Regional Fisheries Research Institute) ;
  • NOGUCHI Tamao (Faculty of Fisheries, Nagasaki University) ;
  • SIMIDU Usio (Research Laboratory No.3, Toyo Suisan Kaisha Ltd.) ;
  • KOGURE Kazuhiro (Ocean Research Institute, University of Tokyo)
  • 발행 : 1996.11.01

초록

The occurrence of TTX in ciliated protozoa was investigated in order to clarify tetrodotoxin (TTX) accumulation mechanisms in marine organisms. Tissue culture bioassay, HPLC, and GC-MS analyses confirmed the occurrence of TTX in Euplotes mutabilis and also in bacteria isolated from the culture medium. Fluorescently labeled bacteria (FLB) were prepared with those bacteria, and predation by E. mutabilis was observed. The results indicated that TTX in bacteria can be transferred to higher trophic levels through the food chain.

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