Korean Journal of Environmental Agriculture (한국환경농학회지)
- Volume 15 Issue 4
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- Pages.399-405
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- 1996
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- 1225-3537(pISSN)
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- 2233-4173(eISSN)
Biological Control of Phytophthora Blight of Red-pepper Caused by Phytophthora capsici.;II. Isolation and Antifungal Activity of the Substances
고추역병균(疫病菌)(Phytophthora capsici)의 생물학적(生物學的) 방제(防除);II. 항균물질(抗菌物質)의 분리(分離) 정제(精製) 및 항균활성(抗菌活性)
- Chang, Yoon-Hee (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
- Chang, Sang-Moon (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
- Choi, Jyung (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
- Lee, Dong-Hoon (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University)
- Published : 1996.12.30
Abstract
In the culture medium, the three antifungal fractions against P. capsici were separated by Sephadex G-25 column chromatography and Silica-gel chromatography. The substance A in white powder and the substance B in sticky oil were isolated by ethyl acetate : acetone mixture(7 : 3), and the substance C in yellow powder was isolated by chloroform : ethyl acetate mixture(95 : 5). The crude extract by ethyl acetate from the culture medium acidified to pH 2 was known to inhibit completely the growth of P. capsici at the level of
고추역병균(疫病菌)인 Phytophthora capsici에 대한 생물학적(生物學的) 방제(防除)를 위하여 Pseudomonas sp. A-183균주(菌株)의 배양액(培養液)으로 부터 3가지 화합물(化合物)을 분리(分離) 정제(精製)한 후(後) 항균활성(抗菌活性)을 조사(調査)하여 미생물농약(微生物農藥)의 개발(開發)에 대한 가능성(可能性)을 검정(檢定)하였다. 배양여액(培養濾液)을 pH 2로 조정하여 ethyl acetate로 추출(抽出)한 경우에 항균활성(抗菌活性)이 가장 높았으며, 조추출물(粗抽出物)
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