Differential Tolerance of Plant Species to Protoporphyrinogen Oxidase-Inhibiting Oxyfluorfen

Protoporphyrinogen oxidase 저해형 Oxyfluorfen에 대한 식물종간 내성차이

The four tolerant and one susceptible plant to oxyfluorfen were selected from 26 species and investigated for the inhibition of protox activity, the PPIX accumulation, and the activity of antioxidative enzymes by oxyfluorfen treatment. When treated, the oxyfluorfen-tolerant plant species showed a less decrease in fresh weight and height than the susceptible one. The susceptible barnyardgrass showed more inhibition of protox activity due to the treatment of oxyfluorfen than the tolerant species. Especially at the concentration of $10^{-6}M$, protox activity of the susceptible barnyardgrass was inhibited completely, but tolerant species maintained $25{\sim}45%$ of the activity. Under the light and dark condition, the susceptible barnyardgrass showed more PPIX accumulation than the tolerant.

26종의 식물종을 공시하여 다양한 선발조건에 의해 oxyfluorfen에 내성 및 감수성식물을 선발하여 이들종에 대한 protox 활성 저해, PPIX 축적정도 및 항산화효소 활성을 체계적으로 연구하여 oxyfluorfen의 내성차이를 구명하고자 하였다. Oxyfluorfen 처리에 따른 protox 활성 저해는 내성식물들 보다 감수성 피에서 컸다. 특히, $10^{-6}\;M$ 이상의 농도에서 감수성 피는 완전히 억제되었으나, 내성식물들은 $25{\sim}45%$의 활성을 보였다. 광 ${\cdot}$ 암조건에서 감수성 피는 내성식물종들 비하여 PPIX 축적량이 훨씬 많았다. Oxyfluorfen 처리후 항산화효소인 MDAR, POX, GR 및 SOD의 활성은 내성식물종들이 감수성 피보다 높았다.