YAKHAK HOEJI (약학회지)
- Volume 40 Issue 2
- /
- Pages.177-182
- /
- 1996
- /
- 0377-9556(pISSN)
- /
- 2383-9457(eISSN)
Determination of Aloesin in Aloe Preparations by HPLC
고속액체크로마토그래피에 의한 알로에 제제 중의 알로에신의 정량
-
Kim, Kyeong-Ho
(College of Pharmacy, Kangwon National University)
;
- Kim, Hyun-Ju (College of Pharmacy, Kangwon National University) ;
-
Park, Jeong-Hill
(College of Pharmacy, Seoul National University)
;
- Shin, Young-Geun (College of Pharmacy, Seoul National University)
- Published : 1996.04.01
Abstract
The contents of aloesin in aloe preparations were determined by HPLC. Aloesin was extracted 3 times with ethanol for 30 minutes. The ethanol extract was concentrated and suspend ed in saturated NaCl aqueous solution and successively partitioned with dichloromethane, n-butanol. Prepared samples were analyzed by HPLC on a reverse column(Inertsil ODS-2). In assay, internal standard was a puerarin and regression of calibration curve was 0.998. Recoveries of aloesin added to aloe preparation were 98~123(%).