대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제26권1호
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- Pages.47-58
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- 1996
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- 1225-2344(pISSN)
한탄바이러스 감염 내피세포에서 부착분자의 발현 (II) -In Situ Hybridization-
Expression of Some Adhesion Molecules on the Cultured Endothelial Cells of Human Umbilical Vein Infected with Hantaan Virus
- 정상인 (중앙대학교 의과대학 미생물학교실) ;
- 신성일 (중앙대학교 의과대학 미생물학교실) ;
- 김기정 (중앙대학교 의과대학 미생물학교실) ;
- 강응택 (중앙대학교 의과대학 내과학교실) ;
- 유석희 (중앙대학교 의과대학 내과학교실) ;
- 최철순 (중앙대학교 의과대학 미생물학교실) ;
- 양용태 (중앙대학교 의과대학 미생물학교실)
- Chung, Sang-In (Department of Microbiology, Chung-Ang University, College of Medicine) ;
- Shin, Sung-Il (Department of Microbiology, Chung-Ang University, College of Medicine) ;
- Kim, Ki-Jeong (Department of Microbiology, Chung-Ang University, College of Medicine) ;
- Kang, Eung-Taek (Department of Internal Medicine, Chung-Ang University, College of Medicine) ;
- Yu, Suk-Hee (Department of Internal Medicine, Chung-Ang University, College of Medicine) ;
- Choi, Chul-Soon (Department of Microbiology, Chung-Ang University, College of Medicine) ;
- Yang, Yong-Tae (Department of Microbiology, Chung-Ang University, College of Medicine)
- 발행 : 1996.06.30
초록
Histopathological vascular changes in hemorrhagic fever with renal syndrome (HFRS) caused by Hantaan virus include increased vascular permeability, disseminated intravascular coagulation, thrombocytopenia and changes in coagulation activity. Although vascular endothelial cells of main target organs such as kidney infected with Hantaan virus are not damaged but swelling of endothelial cells, perivascular exudates and infiltration of mononuclear cells and fresh interstitial hemorrhages are common. However, the pathogenesis of cell infiltration and hemorrhages around vascular endothelial cells are not well understood. Some endothelial cell molecules or vascular adhesins that acts as adhesion moleulces for leukocyte are expressed on endothelial cells close to site of inflammation. However, whether the expression of endothelial adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule (ICAM-1) and endothelial leukocyte adhesion molecule (ELAM) on vascular endothelial cells are increased by infection with Hantaan virus has not been studied. In this study, the relationship between the expression of VCAM-1, ICAM-1 and ELAM and adhesion of mononuclear cells on endothelial cells of human blood vessels infected with Hantaan virus was investigated. The endothelial cells of umbilical vein was passaged three times in culture medium and the monolayered cells were infected with