The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
권호 표지

Isolation and Characterization of Jeju Island Bovine Rotavirus (JBR)

제주도 Bovine Rotavirus의 분리와 그 특성

  • Yu, Jae-Hyeun (Department of Dairy Science, Kon-Kuk University and ARRC) ;
  • Lee, Young-Kun (Department of Veterinary Medicine, Kon-Kuk University and ARRC) ;
  • Ju, Ji-Sun (Department of Veterinary Medicine, Kon-Kuk University and ARRC) ;
  • Kim, You-Seong (Department of Veterinary Medicine, Kon-Kuk University and ARRC) ;
  • Kim, Soon-Jae (Department of Veterinary Medicine, Kon-Kuk University and ARRC) ;
  • Ebina, T. (Miyagi Cancer Center) ;
  • Nakagomi, O. (Faculty of Medicine, Akita University, School of Medicine) ;
  • Urasawa, S. (Sapporo Medical University) ;
  • Taniguchi, K. (Sapporo Medical University) ;
  • Greenberg, Harry B. (School of Medicine, Stanford University)
  • 유제현 (건국대학교 낙농학과 및 동물자원연구센터) ;
  • 이영건 (건국대학교 수의학과 및 동물자원연구센터) ;
  • 주지선 (건국대학교 수의학과 및 동물자원연구센터) ;
  • 김유성 (건국대학교 수의학과 및 동물자원연구센터) ;
  • 김순재 (건국대학교 수의학과 및 동물자원연구센터) ;
  • 해로명탁삼랑 (일본 미야기 암센타) ;
  • 중입치 (일본 아키다대 의학부) ;
  • 포택정삼 (일본 삽뽀로 의과대학) ;
  • 곡구효보 (일본 삽뽀로 의과대학) ;
  • Published : 1996.12.30
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Abstract

Fecal samples of calf diarrhea were taken on farms in Jeju island, rotavirus was isolated and cytopathic effect (CPE) was determined after infection to MA104 cell. Morphological evaluation on electron microscopy proved it as rotavirus. Also, its infection to MA104 cell was reidentified using a fluorescence antibody method. Genotype of Jeju island bovine rotavirus (JBR) analyzed through PAGE was 4: 2: 3: 2 pattern, which was unique in bovine and that analyzed through general PAGE was somewhat different from NCDV, UK, KK3, A5-3A, 61A, B223 and similar to N stool-5, N culture-5 and Kawatabi (Japan). By titration after plaquing, the level was $1-3\;{\times}\;10^6\;PFU/ml$, which was lower than those of NCDV and UK. Electrophoresis analysis of RNA-RNA hybridization, ELISA, and first and second PCR products of VP7 and VP4 in 1% agarose ($TAE+1{\mu}l$ EtBr) revealed that the rotavirus was a serotype of G6P11.

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