Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Lipid Specificity for Membrane Oxidation Catalyzed by Cytochrome c : An EPR Study

  • Published : 1996.03.20
Download PDF
⟨ Previous Next ⟩

Abstract

Decay of the spin label attached to cytochrome c or to stearic acid has been measured by electron paramagnetic resonance (EPR) spectroscopy to monitor membrane oxidation induced by cytochrome c-membrane interaction. Binding of cytochrome c sequestered the acidic phospholipids and membrane oxidation was efficient in the order linoleic oleic>stearic acid for a fatty acid chain in the acidic phospholipids. The spin label on cyt c was destroyed at pH 7 whereas that on stearic acid embedded in the membrane was destroyed at pH 4, presumably due to different modes of cyt c-membrane interaction depending on pH. Interestingly, cyt c also interacts with phosphatidylethanolamine, an electrically neutral phospholipid, to cause rapid membrane oxidation. Both EPR and fluorescence measurements indicated that electrostatic interaction is at least partially responsible for the process.

Keywords

References

  1. Biochimie v.76 Pinheiro, T. J. T.
  2. Eur. Biophys. J. v.18 Hildebrandt, P.;Heimburg, T.;Marsh, D.
  3. FEBS Lett. v.360 de Jongh, H. H. J.;Ritsema, T.;Killian, J. A.
  4. Biochemistry v.15 Birrell, G. B.;Griffith, O. H.
  5. Biochim. Biophys. Acta v.733 Bruch, R. C.;Thayer, W. S.
  6. J. Biol. Chem. v.263 Gupte, S. S.;Hackenbrock, C. R.
  7. Biochim. Biophys. Acta v.1228 Cortese, J. D.;Voglino, A. L.;Hackenbrock, C. R.
  8. J. Biol. Chem. v.270 Rytomaa, M.;Kinnunen, P. K. J.
  9. J. Biochem. Mol. Biol. Min, T.;Park, N.-H.;Park, H. Y.;Hong, S.-J.;Han, S.
  10. Biochim. Biophys. Acta v.468 Brown, L. R.;Wuthrich, K.
  11. Biochim. B iophys. Acta v.835 Goi, F. M.;Ondarroa, M.;Azpiazu, I.;Macarulla, J. M.
  12. Biochim. Biophys. Acta v.878 Valpuesta, J.M.;Ondarroa, M.;Goi, F. M.
  13. Biochim. Biophys. Acta v.857 Szebeni, J.;Toth, K.
  14. Biochim. Biophys. Acta v.1208 Sarti, P.;Hogg, N.;Darley-Usmar, V. M.;Sanna, M. T.;Wilson, M. T.
  15. Proc. Natl. Acad. Sci. USA v.91 Shigenaga, M. K.;Hagen, T. M.;Ames, B. N.
  16. Biophys. J. v.67 Snel, M. M. E.;Marsh, D.
  17. Trends Biochem. Sci. v.17 Milhauser, G. L.
  18. Biochim. Biophys. Acta v.1061 MacDonald, R. C.;MacDonald, R. I.;Menco, B. P. M.;Takeshita, K.;Subbarao, N. K.;Hu, L.-R.
  19. Biochemistry v.26 Mustonen, P.;Virtanen, J. A.;Somerharju, P. J.;Kinnunen, P. J. K.
  20. Biochemistry v.8 Chio, K. S.;Tappel, A. L.
  21. J. Neurosurg. v.76 Nakahara, I.;Kikuchi, H.;Taki, W.;Nishi, S.;Kito, M.;Tonekawa, Y.;Goto, Y.;Ogata, N.
  22. J. Biol. Chem. v.270 Barr, D. M.;Mason, R. P.
  23. Biochemistry v.28 Hildebrandt, P.;Stockburger, M.