A Plasmid of Lactococcus lactis subsp. lactis ML8 Linked with Lactose Metabolism and Extracellular Proteinase

  • LEE, JONG-HOON (Department of Food Technology, Kyonggi University) ;
  • HYONG JOO LEE (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University)
  • 발행 : 1996.12.01

초록

Three distinct plasmids, with approximate molecular weights of 1, 4.5, and 33 megadaltons, were found in Lactococcus lactis subsp. lactis (L. lactis) ML8. Slow acid-producing mutants of L. lactis ML8, isolated by plasmid curing with acriflavine treatment, lacked the 33-megadalton plasmids. The plasmid-cured mutant showed lactose-negative (Lac) characteristics and the alteration of extracellular proteinase pattern. The possible involvement of extracellular proteinase with the 33-megadalton plasmid is highlighted in this research.

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