Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Detection of bovine rotavirus antigen by enzyme linked immunosorbent assay

효소면역법에 의한 소 로타바이러스 항원 검출

  • 안재문 (충청북도 농축산사업소북부지소) ;
  • 유기조 (충청북도 농축산사업소북부지소) ;
  • 이용희 (충청북도 농축산사업소북부지소) ;
  • 이종인 (충청북도 농축산사업소북부지소)
  • Published : 1996.05.01
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Abstract

Enzyme-linked immunosorbent assay (ELISA) was developed to detect rotavirus antigen in fecal samples using VP6-specific monoclonal antibody(2B12). The ELISA for rotavirus antigen detection found to have specificity to all bovine and porcine rotaviruses tested but not to bovine viral diarrhea virus and bovine coronavirus. The ELISA appeared to have similar sensitivity and specificity compared to fluorescence antibody assay(FA) and electropherotyping (PAGE).

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