한국재래간장으로 부터 분리한 Bacillus subtilis CCKS-111이 생성하는 Protease의 특성 및 작용양상

Characteristics and Action Pattern of Pretense from Bacillus subtilis CCKS-111 in Korean Traditional Soy Sauce

  • 최청 (영남대학교 식품가공학과) ;
  • 최광수 (영남대학교 식품가공학과) ;
  • 조영제 (상주산업대학교 식품공학과) ;
  • 임성일 (영남대학교 식품가공학과) ;
  • 김성 (영남대학교 식품가공학과) ;
  • 손준호 (영남대학교 식품가공학과) ;
  • 이희덕 (영남대학교 식품가공학과) ;
  • 김영활 (대구보건전문대학 임상병리과)
  • Choi, Cheong (Dept. of Food Science and Technology, Yeungnam University) ;
  • Choi, Kwang-Soo (Dept. of Food Science and Technology, Yeungnam University) ;
  • Cho, Young-Je (Dept. of Food Engineering, Sangju National Polytechnic University) ;
  • Lim, Sung-il (Dept. of Food Science and Technology, Yeungnam University) ;
  • Kim, Sung (Dept. of Food Science and Technology, Yeungnam University) ;
  • Son, Jun-Ho (Dept. of Food Science and Technology, Yeungnam University) ;
  • Lee, Hee-Duck (Dept. of Food Science and Technology, Yeungnam University) ;
  • Kim, Young-Hwal (Dept. of Clinical Pathology, Daegu Junior College)
  • 발행 : 1996.12.01

초록

한국재래간장으로부터 분리한 Bacillus subtilis CCKS-111이 생성하는 protease 생산의 최적 배양조건은 2% soluble starch, 0.2% peptone, 0.1%(MH$_4$)$_2$S$_2$O$_{8}$ , 0.2% MgSO$_4$, pH 7.0, 35$^{\circ}C$ 에서 24시간 배양했을 때이다. 효소의 최적 작용 pH와 온도는 pH 9.0, 5$0^{\circ}C$였으며 , pH 6.0~11.0의 범위와 5$0^{\circ}C$이상에서 불안정하였다. 금속이온 중 Cu$^{2+}$에 의하여 활성이 증대되었으나 $K^{2+}$, Hg$^{2+}$등에 의하여 효소활성이 저해되었다. Ethylenediamineteraacetic acid 와 phenylmethane sulfonyl fluoride 처리에 의해 활성이 저해되어 금속이온의 영향을 받는 serine protease로 확인되었다. Km값은 2.313$\times$$10^{-4}$ M, V$_{max}$ 값은 39.216$\mu\textrm{g}$/min이었으며, hemoglobin보다 casein을 더 잘 가수분해하였다.

An alkaline pretense Producing microorganism was isolated from Korean traditional soy sauce and identified as Bacillus subtilis CCKS-111. The optimum culture condition of Bacillus subtilis CCKS-111 for the production of alkaline pretense was as follow: 2% soluble starch, 0.2% peptone, 0.1% (NB$_4$)$_2$S$_2$O$_{8}$ , 0.2% MgSO$_4$, pH 7.0, 35$^{\circ}C$ and 24hrs. The optimum pH and temperature for the enzyme activity of alkaline pretense producing Bacillus subtilis CCKS-111 were pH 9.0 and 5$0^{\circ}C$, respectively. The enzyme was relatively stable at pH 6.0~11.0 and at temperature below 4$0^{\circ}C$. The activity of the enzyme was inhibited by $K^{+}$ and Hg$^{2+}$, whereas Cu$^{2+}$ exhibited rather activating effects on the enzyme activity. Ethylenediaminetetraacetic acid and phenylmethanesulfonyl fluoride inhibited the enzyme activity. This indicates that this is serine pretense which requires metal ion group for the enzyme activity. Km value was 2.313$\times$10$^{-4}$ M/L, V$_{max}$ value was 39.216$\mu\textrm{g}$/min. This enzyme hydrolyzed casein more rapidly than the hemoglobin.lobin.

키워드

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