Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
권호 표지

Isolation and Partial Characterization of Bacteriophage from Oyster

생굴로부터 bacteriophage의 분리 및 부분특성

  • 김영희 (동의대학교 미생물학과)
  • Published : 1996.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The bacteriophage from the fresh oyster, Crassostrea Virginica which is specific to the marine bacterium was isolated and characterized. Among the foci different vibrio species and the five different serotypes of Vibrio parahaemolyticus host strains tested, only two strains of the parahaemolyticus possessing K17, K52 antigens were highly sensitive to the phage. The size of the isolated plaque was 0.4mm and the electron microscopic head size of the isolated phage was about 67 nm long and 83 nm wide. PFU/ml was 1.25$\times$ $10^{11}$. The phase was sensitive to chloroform but resistant to acetone or methanol. The assay of the isolated phase nucleic acid was deoxyribonucleic acid. The restriction enzyme pattern showed 14 fragment from Hind III and 4 fragments from Eco R I. Two different antigenic groups showed-similar restriction enzyme patterns.

시판중인 생굴을 대상으로 Vibrio 속을 숙주로 하는 bacteriophage의 분리를 시도하였다. 4 종의 Vibrio 속과 5 혈청형의 Vibrio parahaemolytius를 실험대상으로 한 결과 배brio parahaemolyticus 2 혈청형에서만 bacteriophage가 분리되었다. 분리된 phage의 plaque 크기는 0.4mm였으며 전자현미경적 형태는 미부가 뚜렷하지 않은 육각형의 두부가 관찰되었고 크기는 67nm$\times$83nm 였으며, PFV/ml은 1.25$\times$$10^{11}$이었다. 분리된 phage는 chloro-form에 감수성을 나타내었다. 분리된 phage의 genomic 특성을 규명하기 위하여 핵산을 분리한 결과 DNA로 판명되어졌으며 두 혈청형 모두 제한효소 처리한 결과 Eco R I으로 4부위의 절단양상과 Hind III로부터 14부위 절단양상이 관찰되었다.

Keywords

References

  1. Bacteriophages Adams,M.H.
  2. J. Mol. Biol. v.54 Molecular weights of coliphage and coliphage DNA;Measurement of the molecular weights of bacteriophage T7 by high speed equilibrium centrifugation Bancroft,F.C.;D.Freifelder
  3. Appl.Environ.Microbiol. v.36 Incidence of Vibrio parahaemolyticus bacteriophages and other Vibrio bacteriophages in marine samples Baross,J.A.;Liston,J.;Morita,R.Y.
  4. Applied and Environmental Microbiology v.61 no.3 Multiple Vibrio vulnificus strains in oysters as demonstrated by clamped homogeneous electric field gel electrophoresis Buchrieser,C.;Gagnar,V.V.;Murphree,R.L.
  5. Applied and Environ. Microbiology v.56 no.4 Incidence of urea-hydrolyzing Vibrio parahaemolyticus in Willapa Bay Kaysner,a. charles;Carlos, Abeyta
  6. J. Bacteriol. v.91 Properties of two marine bacteriophages Chen,P.K.;Citarella,R.V.;Salazar,O.;Colwell,R.R.
  7. Journal of Food Protection v.57 no.3 Interaction of Vibrio vulnificus and the Eastem oyster, Crassostrea Virginica Groubert,T.N.;Oliver,J.D.
  8. Journal of Bacteriology v.173 no.12 Properties of the Streptomycete temperate bacteriophage FP 43 Hahn, Donald;R.Margaret;A.Mchenny
  9. Microbiol. Immunol. v.28 no.3 Isolation and characterization of a filamentous phage, Vf 33, specific for Vibrio parahaemolyticus Hatsumi, Taniguchi;Kiyoshi, Sato
  10. J. Korea Soc. Microbiol. v.22 no.1 Studies on the phage of Vibrio parahaemolyticus Ju,J.W.;Rhee,G.H.;Kim,I.L.
  11. Microbiol. Immunol. v.25 Structure of a novel bacteriophage VP 3 for Vibrio parahaemolyticus Koga,T.;Kawata,T.
  12. Environ. Mictobiol. v.36 Ecological relationship between Vibrio parahaemolyticus and agar digesting Vibrios as enidenced by bacteriophage susceptibility patterns Liston,J.A.;Morita,R.Y.
  13. Molecular cloning, a laboratory manual Maniatis,T.;Fritsch,F.H.;J.Sambrook
  14. Journal of Food Protection v.57 no.11 Occurrence of toxigenic Vibrio cholera 01 in oysters in Mobile Bay, Alabama: An ecological investigation Motes,M.A.
  15. Letters in Applied Microbiology v.20 no.4 Effect of chill and freezing temperatures on survival of Vibrio parahaemolyticus inoculated in homogenates of oyster meat Muntada,J.M.
  16. Biken J. v.9 Isolation and properties of bacteriophages of Vibrio parahaemolyticus Nakanishi,H.;Iida,Y.;Maeshima
  17. Applied and Environmental Microbiology Isolation and partial characterization of bacteriophages of the phytopathogen Pseudomonas syringe Nordeen, R. Russel;Michael, K. Morgan
  18. Can. J. Microbiol. v.19 Characteristics of a Vibrio parahaemolyticus bacteriophage isolated from Atlantic coast sediment Sklarow,S.S.;Colwell,R.R.
  19. Journal of Food Protection v.58 no.4 Hot sauce: No elimination of Vibrio vulnificus in oysters Sun,Y.;Oliver,J.D.
  20. J. Bacteriol. v.91 Structure of a marine bacteriophage as revealed by the negative-staining technique Valentine,A.F.;P.K.Chen;R.R.Colwell;G.B.Chapman
  21. Virology v.40 Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification Yamamoto,K.R.;Alberts,B.M.;Benzinger,R.;Lawhore,L.
  22. Applied and Environmental Microbiology v.61 no.1 Viability of Vibrio vulnificus in association with hemocytes of the American oyster Young, Harris;Tamplin,M.L.