Tryptophan 투여가 Reserpine과 식이 탄수화물 수준이 다른 저단백식이를 섭취한 흰쥐의 혈장 아미노산 농도, 간 Cytochrome P450 함량 및 간세표 미세구조에 미치는 영향

The Effect of Tryptophan Administration on the Plasma Free Amino Acid Concentration, Liver Microsomal Cytochrome P450 Content and Cellular Structure of Rats Consumed Reserpine and Low Protein Diet with Different Carbohydrate Contents

The purpose of this experiment was to compare the effects of tryptophan administration on nutritional status of female rats which consumed reserpine and 6% casein diet with different carbohydrate contents(87%, 65%, 44% respective). Final body weight, body weight gain, FER, plasma amino acid concentration and microsomal cytochrome P 450 content in liver were measured and microscopic structure of hepatocytes was observed. In low-protein diet, the higher the carbohydrate content of diet was, the lower the damage was in the rat's liver. Tryptophan administration after dose of reserpine induced more effective recovery from liver damage of rats in high carbohydrate diet group than that in low carbohydrate diet group. In conclusion, the general nutritional assessments such as final body weight and body weight gain provided better estimate of the degree of structural changes in hepatocytes than functional assessment such as plasma amino acid concentration or liver microsomal cytochrome P450.