Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
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Purification and Biochemical Properties of Storage Protein SP-2 in Tobacco Budworm (Helicoverpa assulta Guenee)

담배나방 저장단백질 SP-2의 정제 및 생화학적 특성

  • Published : 1996.06.01
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Abstract

A storage protein(SP-2) was confirmed in haemolymph during larval-pupal-adult development of tobacco burdworm(Helicoverpa assulta Guenee), and its biochemical characteristics were investigated. The titer of SP-2 showed a peak at mature larva, decreased gradually through the late pupal stage, and became undetectable at adult period. As the results from electrophoretic mnysis, SP-2 was confirmed to be glycolipoprotein(M.W. 332kDa) relatively stable to heat( $\leq$ 68$^{\circ}C$ ). This storage protein was determined to be a tetramer composed of a single subunit with MW of 83kDa, and the isoelectric point was 5.7. The amino acid composition of the SP-2 was characterized. It has relatively high content of methionine and histidine, whereas the contents of tyrosine and phenylalanine were relatively low.

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References

  1. Arch. Biochem. Biophys. v.110 Disc electrophoresis of human saliva in polyacrylamide gels Caldwell, R. C.;W. Pigman
  2. J. Insect Physiol. v.12 Haemolymph proteins of Ostrinia nubilaris during diapause and prepupal differentiation Chippendale, G. N.;S. D. Beck
  3. Insect Biochem. Molec. Biol. v.24 Biosynthesis and tissue distribut- ion of four major larval serum proteins during development of Ceratitis capitata(Diptera) Chrysanthis, G.;A. D. Kaliafas;A. C. Mintzas
  4. Insect Biochem. Molec. Biol. v.24 Synthesis of a haemolymph hexamerin by the fat body and testis of Rhodnius prolixus Faria, F. S.;E. S. Garcia;S. Goldenberg
  5. Archs. Insect Biochem. Physiol. v.3 Binding of insecticides to lipophorin and arylphorin, two haemolymph proteins of Heliothis zea Haunerland, N. H.;W. W. Bowers
  6. Insect Biochem. v.20 Fat body heterogeneity during develop- ment of Heliothis zea Haunerland, N. H.;K. K. Nair;W. S. Bowers
  7. Archs. Biochem. Biophysics v.126 Size and charge isomer separation and estimation of molecular weights of protein by disc gel electrophoresis Hedrick, J. L.;A. J. Smith
  8. Archs. Insect Biochem. Physiol. v.10 Storage proteins of the fall webworm, Hyphantria cunea Drury Kim, H. R.;C. S. Kang;R. T. Mayer
  9. Archs. Insect Biochem. Physiol. v.10 Properties, synthesis and accumulation of st orage proteins in Pieris rapae L Kim, H. R.;S. J. Seo;R. T. Mayer
  10. Roux's Archs. Dev. Biol. v.195 Incorporation of calliphorin into the cuticle of the developing blowfly Calliphora vicina Konig, M.;O. P. Agrawal;H. Shenkel;K. Scheller
  11. Archs. Insect Biochem. Physiol. v.20 Storage proteins are present in hemolymph from larvae and adults of the colorado potato beetle Koopmanschap, B.;H. Lammers;S. de Kort
  12. Insect Biochem. v.10 Purification and properties of manducin, an amino acid storage protein of the haemolymph of larval and pupal Manduca sexta Kramer, S. J.;E. C. Mundall;J. H. Law
  13. Nature v.227 Cleavage of structural proteins during assembly of the head of bacteriophage T₄ Laemmli, U. K.
  14. Archs. Insect Biochem. Physiol. v.5 Major plasma proteins of larvae of the southwestern corn borer, Diatraea grandiosella Lenz, C. J.;K. Ventatesh;G. M. Chippendale
  15. Comprehensive Insect Physiology, Biochemistry and Pharmacology v.10 Insect storage proteins Levenbook, L.;Kerkut G. A.(Ed);L. I. Gilbert(Ed)
  16. J. Biol. Chem. v.250 High resolution of two dimensional electrophoresis of proteins O'Farrell, P. H.
  17. Appl. Ent. Zool. v.16 Quantitative changes of storage proteins and vitellogenin during the pupal-adults development in the silkworm, Bombyx mori(Lepidoptera; Bombycidae) Ogawa, K.;S. Tojo
  18. Archs. Insect Biochem. Physiol. v.5 Purification and characterization of three major haemolymph proteins of three major haemolymph proteins of an insect, Calpodes ethlius (Lepidotera, Hesperiidae) Palli, S. R.;M. Locke
  19. Insect Biochem. v.20 Purification and properties of a storage protein from the hemolymph of Rhodnius prolixus Rehn, K. G.;A. L. R. Rolim
  20. Experimentia v.37 The major serum proteins of Dipteran larvae Roberts, D. B.;H. W. Brock
  21. Archs. Insect Biochem. Physiol. v.25 Purification and characterization of a flavin-binding storage protein from thd hemolymph of Galleria mellonella Silhacke, D. L.;S. G. Miller;C. L. Murphy
  22. Insect Biochem. v.13 Arylphorin, a new protein from Hyalophora cecropia; comparisons with callophorin and manducin Telfer, H. W.;P. S. Kein;J. H. Law
  23. Ann. Rev. Entomol. v.36 The function and evolution of insect storage hexamers Telfer, W. H.;J. G. Kunkel
  24. Insect Biochem. Molec. Biol. v.24 Purification and characterization of three storage proteins in the common cutworm, Spodoptera litura Tojo, S.;T. Yoshiga
  25. Insect Biochem. v.10 Storage proteins in the silkworm Tojo, S.;M. Nagata;;M. Kobayashi
  26. J. Inset Physiol v.37 Ultrastructural study of storage protein granules in fat body of the corn earworm, Heliothis zea Wang, Z.;N. H. Haunerland
  27. J. Biol. Chem. v.244 The reliability of molecular weigh determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Weber, K.;M. Osborn
  28. Ann. Rev. Biochem. v.47 Insect plasma proteins Wyatt, G. R.;M. L. Pan
  29. Comp. Biochem. Physiol. v.106B Storage proteins, vitellogenin and vitellin of wild silkorms, Antheraea yamamai, Antheraea pernyi and their hybrids Yokoyama, M. N.;Z. Kajiura;M. Nakagaki;R. Takei;M. Kobayashi;K. Tanaka