The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Determination and Application of 3-Methylhistidine Levels in Urine

뇨(尿)중 3-Methylhistidine 함량의 측정과 이용

  • 정수현 (고려대학교 보건전문대학 식품영양과) ;
  • 서형주 (고려대학교 보건전문대학 식품영양과, 한국식품개발연구원, 공주대학교 식품공학과, 순천향대학교 사회체육학과)
  • Published : 1996.06.01
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Abstract

A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 M sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhlstidine from urine control were 95% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary .3-methylhistidine content of volunteers increased significantly after weight training.

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