Effects of Lipid Peroxidation of LDL and Lp(a)

  • Shim, Young-Hee (Division of Life Science, Kangwon National Univerwity, Chunchon 200-701) ;
  • Kim, Tae-Woong (Division of Life Science, Kangwon National Univerwity, Chunchon 200-701)
  • 발행 : 1996.06.01

초록

Lipopretein(a)[Lp(a)] is a macromolecular complex found in human plasma that combines structural elements composed of LDL and apo(a), and that is associated with premature coronary heart disease and stroke. In this study, various samples which consisted of normal and abnormal LDL and LP(a) were selected for compar-ison. The above samples were incubated with copper in order to oxidize and to compare atheroma formation, in vitro and free radical formation of Lp(a) was decreased compared to purified LDl. And LDL or Lp(a) from a 40 year old donor was higher in the free radical formation than that fro, a 20 years old donor. In order to investigate the macrophage foam cell formation, oxidized LDL of Lp(a) was incubated with human monocyte derived macrophage(HMDM). Oxidized samples enhanced on acceptability f foam cell formation by HMDM were compared to the control group. Also, structural change of LDL and Lp(a) against oxidation times were found from HPLC mapping.

키워드

참고문헌

  1. N. Engl. J. Med. v.320 Beyond cholesterol. Modification of low density lipoprotein that increase its atherogenicity Steinberg,D.;Panthasarathy,S.;Carew,T.E.;Khoo,J.C.;Witztum,J.L.
  2. Heart Disease and Stroke v.1 Lipoproteins Brown,V.B.
  3. Eur. J. Clin. Invest. v.22 Plasma triglyceride and LDL metabolism Caslake,M.J.;Packard,C.J.;Shepherd,J.
  4. Arteriosclerosis v.9 Structure of apo B-100 of human LDL Yang,C.Y.;Gu,Z.W.;Kim,T.W.;Gotto,A.M.;Chan,L.
  5. Thromb Res. v.57 Lipoprotein(a) inhibition of plasminogen activation by tissue-type plasmi-nogen activator Edelberg,J.M.;Gonxalez-Gronow,M.;Pizzo,S.V.
  6. J. Lipid Res. v.28 Human plasma lipoprotein(a) ; structural properties Gaubatz,J.W.;Chari,M.L.;Lnava,M.L.;Guyton,J.R.;Morrisett,J.D.
  7. Proc. Natl. Acad. Sci. USA. v.86 Plasmin catalyzes binding of lipoprotein(a) to immobilized fibrinogen and fibrin Harpel,P.C.;Gordon,B.R.;Parker,T.S.
  8. Plasma lipoproteins Lipoprotein(a) : Structure metabolism and epideminology Morrisett,J.D.;Guyton,J.R.;Gaubatz,J.W.;Gotton,A.M.;Gotto,A.M.Jr(ed.)
  9. Arch. Pathol. Lab. Med. v.112 Lipoprotein(a) : A potential bridge between the fields of atherosclerosis and thrombosis Scanu,A.M.
  10. Annu. Rev. Physiol. v.45 Apolipoprotein B : structural and metabolic heterogenity Kane,J.P.
  11. J. Biol. Chem. v.262 Oxidation of human LDL results in derivatisation of lysine residues of apoprotein B by lipid peroxide decomposition products Steinbrecher,U.P.
  12. Chem. Res Toxicol. v.3 Biochemical, stuctural, and functional properties of oxidized low density lipoprotein Esterbauer,H.;Diaber-Rotheneder,M.;Waeg,G.;Striegl,G.;Jurgens,G.
  13. Biochim. Biophys. Acta v.1081 Oxidation of lipoprotein(a). A comparison with low density lipoproteins Sattler,W.;Kostner,G.M.;Waeg,G.;Esterbauer,H.
  14. Trends in Bioche. Sci. v.17 Molecular flypaper atherosclerosis : structure of the macrophage scavenger receptor Krieger,M.
  15. Proc. Natl. Acad. Sci. USA v.86 LDL undergoes oxidative modification in vivo Palinski,W.;Rosenfeld,M.E.
  16. Biochim. Biophys. Acta v.1044 HDL inhibits the oxidative modification of LDL Parthasarathy,S.;Barnett,J.;Fong,L.G.
  17. Biochem. Soc. Trans. v.18 Endogenous antioxidants and lipoprotein oxidation Esterbauer,H.;Puhl,H.;Tatzber,F.
  18. J. Korean Soc. Food Nutr. v.23 Functional properties of low density lipoprotein and dxidized LDL Kim,T.W.
  19. Clin. Chem. v.36 The unique lipoprotein(a) : properties and immunochemical measurement Albers,J.J.;Marcovina,S.;Lodge,M.S.
  20. J. Clin. Invest. v.90 Apolipoprotein(a) gene accounts for greater than 90% of the variation in plasma lipoprotein Lp(a) concentration Boerwinkle,E.;Leffert,C.C.;Lin,J.;Lackner,C.;Chiesa,G.;Hobbs,H.H.
  21. Genetics of Coronary Heart Disease Polential significance of Lp(a) lipoprotein Dahlen,G.H.;Bearn,A.G.(ed.)
  22. Free radical in aging Yu,B.P.
  23. N. Engl. J. Med. v.320 Beyond cholesterol : Modification of LDL that increase its atherogenecity Steinberg,D.;Carew,T.E.;Witztum,J.L.
  24. J. Cell Sci. Suppl. v.9 Macrophage lipoprotein receptors Ogelman,A.M.;Warden,C.;Harberland,M.E.;Edwards,P.A.
  25. Oxidative Stress Oxidation of LDL in vitro Gebicki,J.M.;Jurgens,G.;Esterbauer,H.