Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Aspergillus neger SFN-416으로부터 생산한 Xylanase II의 분리정제 및 특성

  • 성찬기 (중앙대학교 화학과) ;
  • 이상원 (경상대학교 식품공학과) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 손봉수 (경상대학교 식품공학과)
  • Published : 1996.12.01
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Abstract

Xylanase (EC 3.2.1.8) was purified approximately 4.3 fold from Aspergillus niger SFN-416 by ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAE-Sephacel ion exchange chromatography. Molecular weight of the enzyme was approximately 42,000 daltons. The optimum pH and temperature of the enzyme activity were 5.5 and 50$\circ$C, respectively. The enzyme activity was enhanced by Fe$^{2+}$, and inhibited by Hg$^{2+}$. The activity was decreased by addition of methanol, ethanol, isopropanol and 1-butanol at a concentration of 10%(v/v).

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