한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권6호
  • /
  • Pages.652-656
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

카라기난 분해효소 생산균의 분리, 동정 및 효소생산 최적 조건

  • 양승택 (경성대학교 식품공학과) ;
  • 주동식 (부경대학교 식품공학과) ;
  • 박중제 (부경대학교 식품공학과) ;
  • 이정석 (부경대학교 식품공학과) ;
  • 김명식 (부경대학교 식품공학과) ;
  • 이응호 (부경대학교 식품공학과)
  • 발행 : 1996.12.01
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초록

The 80 strains which produce carrageenan degrading enzyme were isolated from soils, mud, seaweed, marine moluscus and echonodermata samples. Among them, one isolated strain, which showed the highest activity to produce carrageenan degrading enzyme, was used for this study. The isolated strain was identified as Pseudomonas alcaligenes through its morphological, biochemical, and physiological characteristics. The best conditions for enzyme production were 0.7% nutrient broth and 0.2% carrageenan as nitrogen and carbon source, respectively. The optimal pH, NaCl, temperature and culture time for carrageenan degrading enzyme were 7.0, 1.5%, 30* and 96hrs, respectively.

키워드

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