Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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$\alpha$-Amylase와 Glucoamylase를 동시에 분비하는 배수체 재조합효모에 의한 전분기질로 부터의 에탄올 생산

  • Park, Sun-Young (Center for Genetic Engineering Research, The University of Suwon) ;
  • Kim, Min-Soo (Center for Genetic Engineering Research, The University of Suwon) ;
  • Kim, Keun (Center for Genetic Engineering Research, The University of Suwon)
  • 박선영 (수원대학교 유전공학연구소) ;
  • 김민수 (수원대학교 유전공학연구소) ;
  • 김근 (수원대학교 유전공학연구소)
  • Published : 1996.10.01
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Abstract

To improve the fermentation characteristics of the haploid starch-fermenting recombinant yeast strain K114/YIpMS$\Delta$R(LEU2/URA3) secreting both $\alpha$-amylase and glucoamylase was rare-mated with polyploid industrial yeast Saccharomyces sp. K35. The K35 strain had good fermentation-characteristics such as ethanol-tolerance, high temperature and sugar-tolerance, and high fermentation rate. Among the resulting 66 hybrids, the best strain RH51 was selected. The RH51 exhibited amylolytic activity of K114/YIpMS$\Delta$R(LEU2/URA3) as well as ethanol and sugar tolerance of K35. The optimum temperature of hybrid RH51 for starch fermentation was 34$\circ$C which was same as that of K35 but different from that (30$\circ$C) of K114/YIpMS$\Delta$R(LEU2/URA3). The optimum pH was 5.0. The optimum size of inoculum was 2% as the pellet (w/v) of yeast cells. The hybrid strain RH51 produced 7.0% ethanol (w/v) from 20% (w/v) soluble starch while K35 formed almost no ethanol, 0.3% (w/v). RH51 strain produced 7.5% (w/v) ethanol after 8 days in a 2.5 l fermenter containing 800 ml of 20% (w/v) soluble starch. The residual starch content in the fermentation medium was 1.68% (w/v), and therefore almost all the starch was fermented completely.

Keywords

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