Candida parapsilosis 돌연변이주에 의한 Xylitol 생산의 배지조건 최적화

  • 오덕근 (우석대학교 식품공학과) ;
  • 윤상현 (동양제과(주) 기술개발연구소) ;
  • 김정민 (동양제과(주) 기술개발연구소) ;
  • 김상용 (동양제과(주) 기술개발연구소) ;
  • 김정회 (한국과학기술원 생물과학과)
  • 발행 : 1996.08.01

초록

Medium optimization for xylitol production from xylose by Candida parapsilosis ATCC 22019 mutant was performed. Effect of various nitrogen sources on xylitol production was investigatied. Of inorganic nitrogenous compounds, ammonium sulfate was effective for xylitol production and yeast extract was the most suitable orangic nitrogen nutrient for enhancement of xylitol production. Effect of inorganic salts such as KH$_{2}$PO$_{4}$ and MgSO$_{4}$-7H$_{2}$0 on xylitol production was also studied. Optimal medium was selected as xylose of 50 g/l, yeast extract of 5 g/l, (NH4$_{4}$)$_{2}$SO$_{4}$ of 5 g/l, KH$_{2}$PO$_{4}$ of 5 g/l, MgSO$_{4}$-7H$_{2}$O of 0.2 g/l. In a fermentor by using the optimal medium, a final xylitol concentration of 37 g/l could be obtained from 50 g/l of xylose with a xylitol yield of 74% and a xylitol productivity of 0.58 g/1-hr. At 300 g/l xylose, fermentation was also carried out and then a final xylitol concentration of 242 g/l was obtained at 272 hours. It was corresponding to xylitol yield of 80.7% and xylitol productivity of 0.58 g/1-hr.

키워드

참고문헌

  1. J. Food Sci. v.45 A quantitative study of manitol, sorbitol, xylitol, and xylose in wild berries and commercial fruits Makinen.K.K.;E.Soderling.
  2. Food Technol. v.10 Xylitol in sugarfree congections. Pepper.T.;Olinger.P.M.
  3. Food Technol. v.32 Xylitol : Its properties and food applications Emodi,A.
  4. Adv. Food. Res. v.28 Food technological evaluation of xylitol Hyvonen.L.;P.Koivistionen
  5. Biotechnol. Bioeng. v.25 Conversion of pentoses by yeasts Gong.C.S.;T.A.Claypool,L.D.;McCracken.;C.M.Maun.;P.P.Ueng.;G.T.Tsao.
  6. Biotechnol. Bioeng. v.40 Production of xylitol from D-xylose by Candida tropicalis: Optimization of production rate Horitsu.H.;Y.Yahashi;K.Takamizawa.;K.Kawai.;T.Suzuki.;N.Watanabe
  7. Biotechnol. Lett. v.13 Xylitol production by Candida guillermindii: Fermentation behavior Meyrial.V.;J.P.Delgenes.;R.Moletta;J.M.Navarro
  8. J. Ferment. Bioeng. v.67 Conversion of D-xylose into xylitol by immobilized cells of Candida pelliculosa and Methanobacterium sp. HU. Nishio.N.;K.Sugawa;N.Hayase;S.Nagai
  9. Biotechnol. Lett. v.11 D-xylose fermentation by Candida shehatae and Pichia stipitis at low dissolved oxygen levels in fed-batch cultures Du Preez, J.C.;B. van Driessell.;B.A.Prior.
  10. Agric. Biol. Chem. v.40 Xylitol production by Enterbacter liquefaciens Yoshitake,J.;M.Shimamura.;H.Ishizaki;Y.Irie.
  11. Agric. Biol. Chem. v.35 Production of polyalcohol by a Cornyebacterium sp. part I Yoshitake.J.;H.Ohiwa;M.Shimamura
  12. J. Ferment. Technol. v.66 Production of xylitol from D-Xylulose by Mycobacterium smegmatis Izumori,K.;K.Tuzaki
  13. Biotechnol. Lett. v.17 Influence of media composition on xylitol fermentation by Candida guilliermondii usting response surface methodology Roberto.I.C.;S,Sato.;I.M. de Mancilha;M.E.S.Taqueda
  14. J. Ferment. Bioeng. v.67 Xylitol production by a methanol yeast, Candida boidinii (Kloeckera sp.) No. 2201 Vongsuvanlert,V.;Y.Tani.
  15. Biotechnol. Lett. v.17 Effect of nitrogen sources on xylitol production from D-xylose by Candida sp. L-102 Jean.L.;L.B.Tsai.;C.S.Gong.;G.T.Tsao.
  16. Appl. Microbiol. Biotechnol. v.36 D-xylose metabolism in Aureobasidium pullulans: effects of aeration and vitamins Kossaczka.Z.;E.Machova;A. Vojtkoval-Lepsikova
  17. Enzyme Microb. Technol. v.10 Effect of biotin limitation on the conversion of xylose to ethanol and xylitol by Pachy-solen tannophilus and Candida guilliermondii Lee.H.;A.L.Atkin.;F.S.Barbosa;D.R.Dorscheid;H.Schneider
  18. Influence of magnesium concentration on growth, ethanol and xylitol production by Pichia stipitis NRRL Y-7124 v.16 Mahler.G.F.;D.V.Geubel.
  19. 한국산업미생물학회지 v.24 no.2 Candida parapsilosis에 의한 xylitol 생성시 포도당의 영향 오덕근;김종화
  20. Enzyme Microb. Technol v.16 Biochemistry and physiology of xylose fermentation by yeasts Hahn-Hagerdal. B.;H.Jeppsson.;K.Skoog.;B.A.Prior