Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Extracellular Proteinase를 생산하는 효모의 분리동정과 효소의 생산

  • 김창화 (대구대학교 공과대학 식품공학과) ;
  • 이태형 (대구대학교 공과대학 식품공학과) ;
  • 유춘발 (대구대학교 공과대학 식품공학과) ;
  • 진익렬 (경북대학교 자연대학 미생물학과)
  • Published : 1996.08.01
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Abstract

A yeast strain TH65 producing a high level of proteinase under alkaline condition was isolated, and identified as Yarrowia lipolytica by morphological, physiological, and biochemical characteristics. In proteinase productivity, glycerol and glucose among tested carbon sources were very effective, and optimum concentration of glucose was 0.5%. Skim milk was found to be most effective nitrogen source in productivity, and its optimum concentration was 0.6%. But, cysteine, cystine and tryptophane decreased the proteinase productivity. Yeast extract was relatively effective at the range of 0.1-0.5%. The yeast showed maximum production of proteinase at 18$\circ$C, pH 9-11, and cultivation time of 36 hours.

Keywords

References

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