Effects of Polyethylene Glycol on Glucoamylase Production in Recombinant Yeast Culture

Polyethylene Glycol이 재조합 효모 배양에 의한 Glucoamylase 생산에 미치는 영향

The effects of polyethylene glycol (PEG) on glucoamylase production in recombinant Saccharomyces cerevisiae were studied. By PEG addition, the cell growth was not affected. However, the glucoamylase production was increased in all range of PEG molecular weights and concentrations. The optimal molecular weight of PEG was 6000 and the optimal concentration was 1g/L. Using these optimals, the extracellular glucoamylase activity was 23% higher in PEG-containing medium than that in medium without PEG.

Polyethylene glycolCPEG)의 재조합 효모 배양 에서의 세포성장과 glucoamylase 생산에 대한 영향에 대하여 조사하였다. PEG 첨가에 의하여 세포성 장은 영향을 받지 않았으나 재조합 glucoamylase의 생산은 증가되었다. 최척의 PEG 분자량은 6000이었으며 최척의 농도는 1g/L이었다. 이러한 최척의 값을 이용하여 PEG 첨가배지에서 첨가하지 않은 배지보다 약 23%의 세포외 glucoamylase 활성의 증가를 보였다.