Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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A stydy on The Biological Control of Sciarid Fly(Lycoriella sp.) Using Bacillus thuringiensis

Bacillus thuringiensis를 이용한 버섯 파리(Lycoriella sp.)의 생물적 방제에 관한 연구

  • 최광호 (동아대학교 농생물학과) ;
  • 박현철 (동아대학교 농생물학과) ;
  • 박현우 (서울대학교 농생물학과 및 농업 생물 신소재 연구 센터) ;
  • 진병래 (서울대학교 농생물학과 및 농업 생물 신소재 연구 센터) ;
  • 강석권 (서울대학교 농생물학과 및 농업 생물 신소재 연구 센터) ;
  • 손흥대 (동아대학교 농생물학과)
  • Published : 1996.12.01
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Abstract

Thirteen subspecies of Bacillus thuringiensis including B. t. israelensis, B. t. morrisoni PG-14 and B.t. darmstadoemsos known to be toxic to dipteran insects were treated on the mushroom (Flammulina velutipes) compost to estimate the biological control effect of a sciarid fly, Lycoriella sp. According to the results, it was found that there were no significant effects of the tested strains of B, thuringiensis on the control of Lycoriella sp. For further confirmation, larval gut juice of Lycoriella sp. and trypsin were respectively treated into the parasporal crystal proteins of three subspecies of B. t. israelensis, B. t. morrisoni PG-14, and B. t. darmstadiensis. The proteins were separated by SDS-PAGE. According to the results, the major parasporal crystal proteins were respectively produced by B. t. morrisoni as the amount of 52 kd, B. y. israelensis as 37kd and B. t. darmstadiensis as 39kd, but the activity of these proteins could not be unfortunately confirmed in this study.

Keywords

References

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