생명과학회지 (Journal of Life Science)

  • 제6권2호
  • /
  • Pages.120-128
  • /
  • 1996
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

방선균 J-144K가 생산하는 Adenosine Deaminase Inhibitor에 관한 연구

Studies on the Adenosine Deaminase Inhibitor Producing Actinomycetes J144-K

  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 김상웅 (부산대학교 자연과학대학 미생물학과) ;
  • 조영배 (부산대학교 자연과학대학 미생물학과) ;
  • 이인 (인제대학교 기초과학연구소)
  • Jun, Hong-Ki (Department of Microbiology, Pusan National University) ;
  • Kim, Sam-Woong (Department of Microbiology, Pusan National University) ;
  • Jo, Young-Bae (Department of Microbiology, Pusan National University) ;
  • Yeeh, Yeehn (Institute of Basic Sciences, Inje University)
  • 발행 : 1996.06.01
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초록

In the screening of actinomycetes culture filtrate for inhibitor of adenosine deaminase, a novel inhibitor was found in a cultured broth of strain J-144K. The optimum conditions for the adenosine deaminase inhibitor production from the isolated strain J-144K were evaluated. This strain showed the maximum yield of adenosine deaminase inhibitor when grown at pH 7.0 and 30$\circ$C for 60 hours in the medium of 1.0% dextrose, 0.5% yeast extract, 0.5% peptone and 0.1% KH$_{2}$PO$_{4}$ under the aerobic condition. Through the activated charcoal extraction, methanol fractionation, Dowex 50 H$^{+}$ X-8 ion exchange column chromatography, Dowex CI$^{-}$ X-8 ion exchange column chromatography, and Sephadex G-15 gel filtration procedures, this inhibitor was purified with three materials.

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참고문헌

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