Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Bacillus sp. Producing Pullulanase and Culture Conditions for Production and Properties of the Enzyme

Pullulanase를 생산하는 Bacillus 속 세균의 분리와 효소의 최적 생산조건 및 특성

  • 정희경 (동의대학교 미생물학과) ;
  • 김병우 (동의대학교 미생물학과)
  • Published : 1996.06.01
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Abstract

A bacterium producing pullulanase was from soil, and was identified Bacillus cereus and named as Bacillus cereus JK36. The optimal culture conditions for the efficident production of pullulanase from B. cereus JK36 was obtained by cultivating with the medium composed of 1% pullulan, 1% teast extract, 1% bactopeptone, 0.1% NaH$_{2}$PO$_{4}$, 2H$_{2}$O, 0.02% MgSO$_{4}$\ulcorner7H$_{2}$O at 40$\circ$C, initial pH 6.5 for 70 hours. Using the culture supernatant as crude enzyme, the optimal pH and temperature of the pullulanase of this strain were 6.5 and 50$\circ$C. In effect of pH and temperature on the stability of the enzyme, the enzyme was stable in the range of pH6.0$\sim$9.5 and up to 40$\circ$C, respectively. The hydrolysis product on pullulan was mainly maltotriose.

Keywords

References

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