Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effects of Various Quinones (Menadione, Benzoquinone and 2,3-Dimethoxy-1,4-naphthoquinone) on Rat Platelets

Quinones (menadione, benzoquinone, 및 2,3-dimethoxy-1,4-naphthoquinone)의 혈소판 세포독성

  • Published : 1996.12.01
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Abstract

Our previous studies demonstrated that quinone (menadione) is cytotoxic to rat platelets. In an attempt to assess the relative contributions of redox cycling and/or arylation in quinone-induced cytotoxicity, we have studied three quinones with different mechanisms: 2, 3-dimethoxy-1, 4-naphthoquinone (DMNQ; pure redox cycler), menadione (both redox cycler and arylator), and 1, 4-benzoquinone (pure arylator). The order of redox cycling capacity in platelet rich plasma (PRP) isolated from rats was menadione>DMNQ>1, 4-benzoquonone, which was consistent with the previous studies using isolated hepatocytes. 1, 4-Benzoquinone was more toxic to rat platelets than menadione, while DMNQ did not cause cell death at all. Lactate dehydrogenase inhibition studies revealed that 1, 4-benzoquinone inhibited significantly in a time-dependent manner, while menadione and DMNQ did not at all. These results suggested that arylation by quinone compounds might play a critical role in quinone-induced cytotoxicity in rat platelets.

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References

  1. Lactate dehydrogenase, in Method in enzymatic analysis Bergmeyer,H.U.;Bernt,E.;Hess,B.;Bermeyer,H.U.(ed.)
  2. Toxicol. Appl. Pharmacol. Metabolism and cytotoxicity of menadione and its metabolite in rat platelets Chun,J.H.;Seo,D.C.;Chung,S.H.;Lee,J.Y.;Seung,S.A.
  3. Arch. Biochem. Biophys. v.235 Alterations in intracellular thiol homeostasis during the metabolism of menadione by isolated rat hepatocytes DiMonte,D.;Ross,D.;Bellomo,G.;Eklow,L.;Orrenius,S.
  4. Arch. Biochem. Biophys. v.235 Menadione-induced cytotoxicity with protein thiol oxidation and alteration in intracellular Ca homeostasis DiMonte,D.;Bellomo,G.;Thor,G.;Nicotera,P.;Orrenlus,S.
  5. Chem. Biol. Interact. v.65 Redox cycling and sulphydryl arylation;their relative importance in the mechanism of quinone cytotoxicity to isolated hepatocytes Gant,T.W.;Rao,D.N.R.;Mason,R.P.;Cohen,G.M.
  6. Toxicol. Appl. Pharmacol. v.138 The mechanism of menadione-induced cytotoxicity in rat platelets Kim,K.A.;Lee,J.Y.;Park,K.S.;Kim,M.J.;Chug,J.H.
  7. Arch. Biochem. Biophys. v.134 The reaction of benzoquinone with amines and proteins Morison,M.;Steele,W.;Danner,D.J.
  8. Biochem. Pharmacol. v.44 Cytotoxic effects of phenyl hydroquinone and some hydroquinones on isolated rat hepatocytes Nakagawa,Y.;Moldeus,P.
  9. Thromb. Res. v.30 An improved method for washing human platelets with prostagladin Radomski,M.;Moncada,S.
  10. Chem. Biol. Interact. v.55 Interaction of menadione(2-methyl-1.4-naphthoquinone) with glutathione Ross,D.;Thor,H.;Orrenius,S.;Moldeus,P.
  11. Arch. Biochem. Biophys. v.248 The role of oxidative processes in the cytotoxicity of substitute 1,4-naphthoquinones in isolated hepatocytes Ross,D.;Thor,H.;Threadgill,M.D.;Sandy,M.S.;Smith,M.T.;Moldeus,P.;Orrenius,S.
  12. Arch. Biochem. Biophys. v.251 Quinone toxicity in hepatocytes without oxidative stress Rossi,L.;Moore,G.A.;Orrenius,S.;O'Brien,P.J.
  13. J. Biol. Chem. v.257 The metabolism of menadione (2-methyl-1,4-naphthoquinone) by isolated hepatocytes Thor, H.;Smith,M.T.;Hartzell,P.;Bellomo,G.;Jewell,S.A.;Orrenius,S.
  14. Chem. Biol. Interact. v.61 Reactivity of thiols towards derivatives of 2- and 6-methyl-1,4-naphthoquinone bioreductive alkylating agent Wilson,I.;Wardman,P.;Lin,T.;Sartorelli,A.C.