Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effect of Fumonisin B1 on the Bacterial Virus Multiplication

세균 바이러스 증식에 대한 Fumonisin B1의 영향

  • 이길수 (한림대학교 자연과학대학 생물학과)
  • Published : 1996.06.01
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Abstract

The effect of Fumonisin B1, a mycotoxin produced by Fusarium moniliforme on bacterial viruses P1 and Lambda, was investigated by the virus plaque assay. Fumonisin B1 inhibited the P1 viral multiplication in the concentration range from $100{\mu}g$/ml to $400{\mu}g$/ml. The inhibition was Fumonisin B1 concentration-dependent. Another bacterial virus Lambda multiplication was also inhibited by lower concentration of Fumonisin B1 ($10{\mu}g$/ml~$50{\mu}g$/ml). This inhibition was dependent on Fumonisin B1 and on virus-Fumonisin B1 reaction time. Sensitivity of bacteriophage Lambda to Fumonisin B1 was higher than that of P1 virus. Lambda vital DNA was treated in vitro with Fumonisin B1 at various concentration. Significant DNA fragmentation by Fumonisin 191 was observed in the agarose gel electrophoresis. Lambda viral DNA was partially digested even in the Fumonisin B1 $10{\mu}g$ and the level of its fragmentation was dependent on Fumonisin B1 amount up to $30{\mu}g$ per assay.

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References

  1. Carcinogenesis v.15 no.2 Fumonisin B1 dosimetry in relation to cancer initiation in rat liver Gelderblom,W.C.A.(et al.)
  2. Appl. Environ. Microbiol. v.54 Fumonisins-novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme Gelderblom,W.C.A.(et al.)
  3. Korean J. Toxicol. v.10 no.2 Fusarium moniliforme 대사산물 Fumonisin B1의 세포 및 유전독성 Lee,K.S.
  4. J. Biol. Chem. v.268 no.36 Fumonisin B1 Inhibits Sphingosine (Sphinganine) N-Acyltransferase and de Novo Sphingolipid Biosynthesis in Cultured Neurons in situ Merrill,A.H.(et al.)
  5. A Short Course in Bacterial Genetics Miller,J.H.(et al.)
  6. Proc. Soc. Exp. Biol. Med. v.202 Neurotransmitters in Rats Fed Fumonisin B1 Porter,J.K.(et al.)
  7. J. Biol. Chem. v.269 no.5 Disruption of Sphingolipid Metabolism and Stimulation of DNA Synthesis by Fumonisin B₁ Schroeder,J.J.(et al.)
  8. International J. Food Microbiol. v.18 Fumonisins Scott,P.M.
  9. Korean J. Toxicol. v.11 no.1 Disruption of Sphingolipid Metabolism as a Potential Mechanism of Fumonisin Inhibition of Cell Growth in LLC-PK₁ Cells Yoo,H.S.