한국동물학회지 (The Korean Journal of Zoology)
- 제39권4호
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- Pages.393-399
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- 1996
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- 0440-2510(pISSN)
전사개시전 복합체에서 TBP, GAL4-AH, TFIIB의 상호작용에 대한 Zero-Length Crosslinking 실험
Zero-Length Crosslinking Study on Interactions of TBP, GAL4-AH, and TFIIB in the Preinitiation Complex
초록
전사개시전 복합체(preinitiation complex)에서 단백질간의 상호작용을 연구하시 위해 zero-Iength croessinking방법을 이용하였다. DNA template가 결합한 금속 지지체를 이용하여 in vitro에서 전사개시전 복합체를 형성시키고, 이렇게 만든 복합체를 1-ethyl-3-(3-dimethylaminopropyl) carbodlimide(EDC)로 croessinking시켰다.
A zero-Iength croessinking procedure for studying protein-protein interactlons in preinitiation complex has heen developed. Preinltiadon complexes were assembled with immobilized DNA templates coupled to metal beads. Pudfied complexes were dIrectly crosslinked by 1-ethyl-3.(3-dimethylaminopropyl)carbodlimide (EDC). The reaction was stopped by addition of