한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제19권4호
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- Pages.259-264
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- 1996
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- 1226-5284(pISSN)
소 분할 초기배와 호르몬, 난관상피세포 및 난구세포와의 공배양에 따른 체외발생율에 관한 연구
Studies on in vitro Developmental Rate of Bisected Bovine Embryos Co-Cultured in TCM-199 Medium Containing Hormones, Oviductal Epithelial Cells and Cumulus Cells
초록
This study was carried out to investigate on the survival rates and in vitro developmental rates of bisected bovine embryos were by manipulator and micropipette. Bisected embryos were co-cultured in 20% FCS(v/v)+TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells 0 to 72 hrs after bisection. Survival rate and in vitro fertilization rate were defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro developmental rate of biseciton embryos co-cultured in 20% FCS+TCM-199 medium containing PMSG, hCG, PMSG+hCG, hCG+
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