Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지

Expression of Lac Z Gene in Young Chick Gonad by the Transtected Primordial Germ Cell Injection

Lac Z 유전자가 전이된 원시생식세포 주입에 의한 병아리 생식기내 유전자 발현

  • 한재용 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 서동삼 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 홍영호 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 정동기 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 최강덕 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 신영수 (신구전문대 축산과)
  • Published : 1996.06.01
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Abstract

Primordial germ cells (PGCs) were manipulated as part of the system to produce transgenic chickens. PGCs were isolated from the germinal crescent of developmental stage 6 to 8 donor emhryos of the Korean Native Ogol Chickens (KNOC). These PGCs were transfected with plasmid DNA containing the lac Z gene by liposome mediated transfection methods. The lac Z gene was transfected and expressed in the PGCs. These transfected PGCs were injected into the germinal crescent of White Leghorn embryos (stage 6 to 8). The injected transfected PGCs migrated via the circulatory system into the future gonad and expression observed in the gonads of 3 day old chick. Of the 47 embryos and 3 day old chickens, one positive PGCs gonad from sacrificed young chickens was detected by appearance of blue cells. Plasmid DNA with the foreign gene was incorporated into the population of germ cells in the gonad. These results demonstrate that PGCs can he transfected and then transferred for colonization into the gonad, and show the potential to ultimately manipulate the genetic material of the chicken gernline.

Keywords

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