Parasites, Hosts and Diseases
- Volume 34 Issue 4
- /
- Pages.215-224
- /
- 1996
- /
- 2982-5164(pISSN)
- /
- 1738-0006(eISSN)
Localization of cytoskeletal proteins in Cryptosporidium parvum using double immunogold labeling
이중면역황금표지법을 이용한 작은와포자충의 세포골격 단백질 분포 관찰
Abstract
actin and some actin binding proteins such as tropomyosin, α-actinin and troponin T were localized by simultaneous double immunogold labeling in several developmental stages of Cryptosporidium parvum. All of the observed developmental stages have many paricles of tropomyosin and actin around pellicle and cytoplasm. Tropomyosin was labeled much more than the actin when these two proteins were labeled simultaneously. And α-actinin was labeled mostly in the pellicle, but troponin T labeling weas very rarely observed. From this study it was suggested that tropomyosin seemed to be one of the major proteins of C. parvum, so it must be playing important roles in C. parvum.
작은와포자충의 세포골격 단백질 분포를 알아보기 위하여 actin, tropomyosin,